食源性变形杆菌氨基糖苷类修饰酶基因及16SrRNA甲基化酶基因研究  

Epidemiological study of the genes conferring aminoglycoside and 16S rRNA methylase in foodborne Proteus

作　　者：郭玉梅[1] 张慧贤[2] 秦丽云[1] 剧慧栋[1] 

机构地区：[1]石家庄市疾病预防控制中心微生物检验所,河北石家庄050011 [2]河北省血液中心检验科,河北石家庄050011

出　　处：《中国食品卫生杂志》2015年第6期611-613,共3页Chinese Journal of Food Hygiene

基　　金：石家庄市科学技术研究与发展指导计划(121461733)

摘　　要：目的了解食源性变形杆菌的氨基糖苷类耐药基因情况。方法收集2011—2014年石家庄市售各类食品中分离到的对阿米卡星和庆大霉素至少有一种耐药的变形杆菌124株,用PCR、测序和基因库在线比对方法分析6种氨基糖苷类修饰酶基因与3种16S r RNA甲基化酶基因。结果 124株变形杆菌中氨基糖苷类修饰酶耐药基因aac C2、aac A4、aad A1和aph A6的检出率分别为95.2%(118/124)、80.6%(100/124)、73.4%(91/124)和5.6%(7/124);16S r RNA甲基化酶耐药基因rmt B检出率为87.1%(108/124)。aac C1、aad B、arm A和rmt C基因均未检出。结论 aac C2型氨基糖苷类修饰酶基因与rmt B型16S r RNA甲基化酶基因流行是食源性变形杆菌对氨基糖苷类抗生素耐药的重要原因。Objective To understand the prevalence of genes conferring aminoglycoside resistance in foodborne strains of Proteus. Methods 124 strains of Proteus were collected from food samples of markets in Shijiazhuang from 2011 to 2014, which resisted toamikacin （AK） or/and gentamicin （CN）. 6 kinds of aminoglycoside modifying enzyme genes （AMEs） and 3 kinds of 16S rRNA methylase genes were analyzed by PCR. Results The prevelence of the AMEs genes , aacC2, aacA4, aadA1 and aphA6, was 95.2% （118/124） , 80. 6% （100/124）, 73.4% （91/124） and 5.6% （7/124） respectively. The prevenlence of 16S rRNA methylase gene rmtB was 87.1% （108/124）. Other genes conferring aminoglyeoside resistance, such as aacC1, aadB, armA and rmtC gene were not detected. Conclusion The spread of AMEs gene aacC2 and 165 rRNA methylase gene rmtB plays an important role in aminoglycosides resistance of foodborne Proteus.

关 键 词：变形杆菌 氨基糖苷类 耐药基因 氨基糖苷类修饰酶 16S r RNA甲基化酶 食源性致病菌 

分 类 号：R446.5[医药卫生—诊断学]