猪带绦虫重组BCG-TSOL18疫苗诱导小鼠免疫应答的动态观察  

Dynamic observation of immune responses induced in mice by immunization with a recombinant BCG- TSOL18 vaccine of Taenia solium

作　　者：杨凤娇[1] 江楠[1] 周泠[2] 刘晖[1] 王灵军[1] 周必英[1] 

机构地区：[1]遵义医学院寄生虫学教研室,563000 [2]遵义医学院附属医院中医肛肠科

出　　处：《中华地方病学杂志》2015年第12期878-883,共6页Chinese Journal of Endemiology

基　　金：贵州省科技厅社会发展攻关项目（黔科合SY字[2011]3038号）

摘　　要：目的观察猪带绦虫重组（r）BCG-TSOL18疫苗免疫小鼠后，诱导产生体液和细胞免疫应答的变化。方法按体质量采用随机数字表法将80只昆明小鼠分为4组：rBCG．TSOL18腹腔注射组f用5X10。克隆形成单位（CFU）猪带绦虫rBCG-TSOL18疫苗腹腔注射免疫小鼠]、rBCC-TSOL18口服灌胃组（用4×10^8CFU猪带绦虫rBCC-TSOL18疫苗口服灌胃免疫小鼠）；卡介苗（BCG）对照组[用5×10^6CFU的BCG腹腔注射免疫小鼠]；磷酸缓冲液（PBS）对照组（PBS腹腔注射小鼠），每组20只。在免疫后0、2、4、6、8周采集小鼠（n=4）眼静脉血．分离血清，采用酶联免疫吸附试验（ELISA）检测小鼠血清特异性I如和IgG2a水平；无菌取脾，制备脾淋巴细胞悬液，收集脾淋巴细胞培养上清液，采用CCK-8法检测脾淋巴细胞增殖水平；ELISA法检测脾淋巴细胞培养上清液白细胞介素（IL）．2和IL-4水平。结果rBCG-TSOL18腹腔注射组和rBCG—TSOL18口服灌胃组小鼠血清特异性IgG水平在免疫后2—8周升高，6周时两组达较高水平（0．310±0．022、0．356±0．026），与同组0周时及同时间BCG和PBS对照组（0．054±0．005、0．057±0．006、0．093±0．014、0．085±0．010）比较，差异有统计学意义（P均〈0．05）；IgG2a水平在免疫后2～8周升高，6周时两组达较高水平（0．965±0．031、1．144±0．049），与同组0周时及同时间BCG和PBS对照组（0．102±0．014、0．093±0．012、0．115±0．012、0．103±0．013）比较，差异有统计学意义（P均〈0．05）；小鼠脾淋巴细胞增殖水平在免疫后2～8周升高，6周时两组达较高水平（0．524±0．032、0．755±0．016），与同组0周时及同时间BCG和PBS对照组（0．301±0．018、0．305±0．020、0．362±0．033、0．334±0．027）比较，差异有统计学意义（P均〈0．05）；脾淋巴细胞上清液中IL-2和IL-4水平分别在免疫后2～8周和4，6周升高，分别在6�Objective To dynamically observe humoral and cellular immune responses induced in mice by immunization with a recombinant BCG-TSOL18 vaccine of Taenia solium. Methods Totally 80 Kunming mice were divided into 4 groups by using random number table according to body mass, 20 mice in each group： rBCG- TSOL18 intraperitoneal injection group [mice were vaccinated with 5 × 10^6 colony forming units （CFU） recombinant BCG-TSOL18 vaccine of Taenia solium through intraperitoneal injection], rBCG-TSOL18 intragastric administration group （mice were vaccinated with 4 × 10^8 CFU recombinant BCG-TSOL18 vaccine of Taenia solium through intragastric administration）, BCG control （mice were vaccinated with 5 × 10^6 CFU BCG through intraperitoneal injection）, PBS control （mice were vaccinated with PBS through intraperitoneal injection）. Zero, 2, 4, 6 and 8 weeks after immunization, eye blood was collected and serum w as separated. Levels of specific IgG and IgG2a were detected by enzyme linked immunosorbent assay （ELISA）. Proliferation level of spleen lymphocytes was detected by CCK-8. Levels of interleukin-2 （IL-2） and IL-4 were determined by ELISA. Results The level of specific IgG in rBCG-TSOL18 intraperitoneal injection group and rBCG-TSOL18 intragastric administration group increased from 2 to 8 weeks, and reached the highest level by the 6th week （0.310 ± 0.022, 0.356 ± 0.026）. Compared with 0 week in the same group, BCG and PBS control group of the same time periods （0.054 ± 0.005, 0.057 ± 0.006, 0.093 ± 0.014, 0.085 ± 0.010）, there were statistically significant differences （all P 〈 0.05）. The level of specific IgG2a increased from 2 to 8 weeks, and reached the highest level by the 6th week （0.965 ± 0.031, 1.144 ± 0.049）. Compared with 0 week in the same group, BCG and PBS control group of the same time periods （0.102 ± 0.014, 0.093 ± 0.012, 0.115 ± 0.012, 0.103 ± 0.013）, there were statistically significant differences （all P 〈 0.05）. The proliferation lev

关 键 词：猪带绦虫 重组BCG—TSOL18疫苗 免疫应答 

分 类 号：R392[医药卫生—免疫学]