纳美芬对大鼠脑缺血再灌注损伤的影响  

Effect of nalmefene on cerebral ischemia-reperfusion injury in rats

作　　者：王志仪[1] 李文瑶[1] 陶国才[2] 

机构地区：[1]四川省肿瘤医院麻醉科,成都市610041 [2]第三军医大学附属西南医院手术麻醉科

出　　处：《中华麻醉学杂志》2015年第10期1189-1191,共3页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（81171034）

摘　　要：目的 探讨纳美芬对大鼠脑缺血再灌注损伤的影响.方法 雄性SD大鼠48只,3～4月龄,体重220～260 g,采用随机数字表法分为4组（n=12）：空白对照组（C组）、假手术组（S组）、脑缺血再灌注组（I/R组）和纳美芬组（N组）.采用夹毕双侧颈总动脉20 min再灌注的方法制备大鼠脑缺血再灌注损伤模型.C组不做任何处理;S组暴露双侧颈总动脉20 min后缝合;N组于再灌注即刻腹腔注射纳美芬0.1 mg/kg.于再灌注6、24和72 h时采集静脉血标本,采用ELISA法测定血浆S-100β蛋白和神经元特异性烯醇化酶（NSE）浓度.于最后1次取血后,断头取脑,采用ELISA法测定脑组织IL-1β和TNF-α含量.结果 与C组比较,S组、I/R组再灌注各时点血浆S-100β蛋白和NSE浓度、脑组织TNF-α和IL-1β含量升高（P＜0.01）;与S组比较,I/R组再灌注各时点血浆S-100β蛋白和NSE浓度、脑组织TNF-α和IL-1β含量升高（P＜0.01）;与I/R组比较,N组再灌注各时点血浆S-100β蛋白和NSE浓度、脑组织TNF-α和IL-1β含量降低（P＜0.01）.结论 纳美芬可减轻大鼠脑缺血再灌注损伤.Objective To investigate the effect of nalmefene on the cerebral ischemia-reperfusion （I/R） injury in rats.Methods Forty-eight male Sprague-Dawley rats, aged 3-4 months, weighing 220260 g, were randomly allocated to control group （group C）, sham operation group （group S）, cerebral I/R group （group I/R）, or nalmefene group （group N） using a random number table, with 12 rats in each group.Cerebral I/R was induced by occlusion of bilateral common carotid arteries for 20 min followed by reperfusion.Group C received no treatment.Group S underwent 20 min exposure of bilateral common carotid arteries and then received suture.In group N, nalmefene 0.1 mg/kg was injected intraperitoneally immediately after reperfusion.At 6, 24 and 72 h of reperfusion, venous blood samples were collected for determination of the concentrations of S-100β protein and neuron-specific enolase （NSE） in plasma by enzyme-linked immunosorbent assay.After the last blood sampling, the rats were sacrificed, and brains were removed for determination of interleukin-1beta （IL-1β） and tumor necrosis factor-alpha （TNF-α） contents in brain tissues by enzyme-linked immunosorbent assay.Results Compared with group C, the plasma S-100β protein and NSE concentrations at each time point of reperfusion, and TNF-α and IL-1βcontents in brain tissues were significantly increased in S and I/R groups （P〈0.01）.Compared with group S, the plasma S-100β protein and NSE concentrations at each time point of reperfusion, and TNF-α and IL-1β contents in brain tissues were significantly increased in group I/R （P〈0.01）.Compared with group I/R, the plasma S-100β protein and NSE concentrations at each time point of reperfusion, and TNF-α and IL-1β contents in brain tissues were significantly decreased in group N （P 〈 0.01）.Conclusion Nalmefene can mitigate cerebral I/R injury in rats.

关 键 词：纳曲酮 再灌注损伤 脑 

分 类 号：R614[医药卫生—麻醉学]