七氟醚对失血性休克猪肠黏膜水通道蛋白8表达的影响  

Effect of sevoflurane on expression of aquaporin 8 in intestinal mucosa in a pig model of hemorrhagic shock

作　　者：王丽晶[1] 王丽斯[2] 孙莹杰[1] 宋丹丹[1] 刁玉刚[1] 张铁铮[1] 

机构地区：[1]沈阳军区总医院麻醉科,110016 [2]内蒙古自治区医院麻醉科

出　　处：《中华麻醉学杂志》2015年第10期1264-1266,共3页Chinese Journal of Anesthesiology

基　　金：“十二五”全军医学科研重点项目（BWS12J008）

摘　　要：目的 评价七氟醚对失血性休克猪肠黏膜水通道蛋白8（AQP8）表达的影响.方法 健康成年巴马小型猪24头,雌雄不拘,体重22～ 25 kg,采用随机数字表法分为假手术组（S组）、失血性休克组（HS组）和七氟醚组（PS组）,每组8头.S组麻醉后经股动脉和颈内静脉置管;HS组麻醉置管后制备大鼠失血性休克模型;PS组于制备失血性休克模型成功后吸入2％七氟醚30 min.于麻醉前（T0）、失血性休克后0.5、1、1.5、2、3和4h（T1-6）时取颈内静脉血样,采用ELISA法检测血清D-乳酸、肠脂肪酸结合蛋白（I-FABP）的浓度;失血性休克4h后放血处死动物取肠组织制作病理切片,观察病理学结果;称湿重和干重,计算肠水含量,采用ELISA法检测肠黏膜AQP8的表达.结果 与S组比较,HS组和PS组血清D-乳酸、I-FABP浓度、肠黏膜AQP8表达水平及肠水含量升高（P＜0.05）;与HS组比较,PS组上述指标降低（P＜0.05）.PS组肠组织病理学损伤较HS组明显减轻.结论 七氟醚可通过抑制AQP8表达降低肠黏膜水肿,从而减轻失血性休克猪肠黏膜损伤.Objective To evaluate the effect of sevoflurane on the expression of aquaporin 8 （AQP8） in the intestinal mucosa in a pig model of hemorrhagic shock.Methods Twenty-four Bama miniature pigs of both sexes, weighing 22-25 kg, were randomly divided into 3 equal groups using a random number table： sham operation group （group S）, hemorrhagic shock group （group HS） and sevoflurane group （group PS）.The femoral artery and jugular vein were cannulated for blood pressure monitoring, blood-letting, and blood sampling in anesthetized pigs.Hemorrhagic shock was induced by withdrawing blood from the right femoral artery.Hemorrhagic shock was induced after cannulation in group HS.In group PS, 2% sevoflurane was inhaled for 30 min after the model of hemorrhagic shock was successfully established.Before anesthesia, and at 0.5, 1, 1.5, 2, 3 and 4 h after hemorrhagic shock, blood samples were collected from the jugular vein for determination of serum D-lactic acid and intestinal fatty acid-binding protein （I-FABP） concentrations.The animals were sacrificed at 4 h after hemorrhagic shock, and the intestinal specimens were obtained for microscopic examination and for determination of AQP8 expression in the intestinal mucosa （by enzyme-linked immunosorbent assay）.The intestinal water content was calculated.Results Compared with group S, the serum D-lactic acid and I-FABP concentrations, AQP8 expression, and intestinal water content were significantly increased in HS and PS groups （P〈0.05）.Compared with group HS, the serum D-lactic acid and I-FABP concentrations, AQP8 expression, and intestinal water content were significantly decreased in group PS （P〈0.05）.The pathological changes of intestinal tissues were significantly reduced in group PS as compared with group HS.Conclusion Sevoflurane can decrease the intestinal mucosal edema through inhibiting AQP8 expression, thus reducing hemorrhagic shockinduced damage to the intestinal mucosa in pigs.

关 键 词：麻醉药 吸入 休克 出血性 肠黏膜 水孔蛋白质类 

分 类 号：R614[医药卫生—麻醉学]