脂联素对肾缺血再灌注小鼠肾纤维化的影响  

Effect of adiponectin on renal fibrosis in mice subjected to renal ischemia-reperfusion

作　　者：杨智慧[1] 伍辉萍[1] 林森[1] 黄振兴[1] 黄腾[1] 仲吉英[1] 杨承祥[1] 周俊[1] 

机构地区：[1]佛山市第一人民医院麻醉科,528000

出　　处：《中华麻醉学杂志》2015年第10期1267-1270,共4页Chinese Journal of Anesthesiology

摘　　要：目的 脂联素对肾缺血再灌注小鼠肾纤维化的影响.方法 雄性C57BL/6小鼠48只,8周龄,体重20～25 g,采用随机数字表法分为4组（n=12）：对照组（C组）、脂联素组（APN组）、缺血再灌注组（I/R组）和缺血再灌注＋脂联素组（I/R＋APN组）.采用阻断左侧肾蒂30 min再灌注法制备肾脏缺血再灌注损伤模型.I/R＋APN组于缺血前15 min和再灌注每24 h腹腔注射APN蛋白球状片段5 μg/g,连续2周;C组分离左侧肾脏,但不夹闭肾蒂,C组和I/R组与相应时点腹腔注射等量生理盐水.APN组分离左侧肾脏,但不夹闭肾蒂,给药方法同I/R＋APN组.于再灌注后48 h时,取左侧肾组织,行肾小管损伤评分,采用TUNEL法检测凋亡指数（AI）.再灌注2周时取肾组织,分别行天狼星红饱和苦味酸（Sirus red）染色和蛋白定量,计算肾间质胶原沉积面积占视野内肾小管间质总面积的百分比（Sirus red面积百分比）,评估左侧肾脏纤维化程度;Western blot法测定Ⅰ型胶原（COL-Ⅰ）、α-平滑肌动蛋白（α-SMA）和纤维连接蛋白（FN）的表达水平.结果 与C组比较,I/R组和APN＋I/R组肾小管损伤评分、AI和Sirus red面积百分比升高,肾组织COL-Ⅰ、α-SMA和FN表达上调（P＜0.05）,APN组上述指标差异无统计学意义（P＞0.05）;与I/R组比较,APN＋I/R组肾小管损伤评分、AI和Sirus red面积百分比降低,肾组织COL-Ⅰ、α-SMA和FN表达下调（P＜0.05）.结论 脂联素可减轻肾缺血再灌注小鼠肾纤维化.Objective To evaluate the effect of adiponectin on renal fibrosis in mice subjected to renal ischemia-reperfusion （I/R）.Methods Forty-eight male C57BL/6 mice, aged 8 weeks, weighing 20-25 g, were randomly divided into 4 groups （n =12 each） using a random number table： control group （group C）, adiponectin group （group APN） and group I/R.Renal ischemia was induced by occlusion of the left renal pedicles for 30 min with atraumatic microclips followed by reperfusion.Globular domain of adiponectin 5 μg/g was injected intraperitoneally at 15 min before ischemia and every 24 h during reperfusion for 2 consecutive weeks in group I/R＋APN.In group C, the left renal pedicles were only isolated, but not occluded, and the equal volume of normal saline was given at the corresponding time point in groups C and I/R.In group APN, the left renal pedicles were only isolated, but not occluded, and the medication was similar to those previously described in group I/R＋APN.At 48 h of reperfusion, 6 mice were sacrificed in each group, the left kidneys were removed for detection of cell apoptosis （using TUNEL） , and the damage to the renal tubules was scored.Apoptosis index （AI） was calculated.The left 6 mice in each group were sacrificed at 2 weeks of reperfusion, and the left kidneys were obtained, the degree of fibrosis was evaluated using Sirus red staining, and the expression of type Ⅰ collagen （Col-Ⅰ）, a-smooth muscle actin （α-SMA）, and fibronectin （FN） was detected by Western blot.Results Compared with group C, the renal tubular damage score, AI and degree of fibrosis were significantly increased, and the expression of COLⅠ , α-SMA and FN was up-regulated in I/R and APN＋I/R groups （P〈0.05）, and no significant change was found in the parameters mentioned above in group APN （P〉0.05）.Compared with group I/R, the renal tubular damage score, AI and degree of fibrosis were significantly decreased, and the expression of COL-Ⅰ , α-SMA and FN was down-regulated in group AP

关 键 词：脂联素 肾脏 再灌注损伤 纤维化 

分 类 号：R692[医药卫生—泌尿科学]