羟考酮预处理对大鼠肠缺血再灌注诱发肝损伤的影响及不同阿片受体在其中的作用  

Effect of oxycodone preconditioning on liver injury induced by intestinal ischemia-reperfusion in rats and the role of different opioid receptors

作　　者：靖国庆 范钧钊 任瑶瑶[1] 陈刘芳[1] 张宗泽[1] 王焱林[1] 吴云[1] 

机构地区：[1]武汉大学中南医院麻醉科,430071

出　　处：《中华麻醉学杂志》2015年第10期1271-1273,共3页Chinese Journal of Anesthesiology

摘　　要：目的 评价羟考酮预处理对大鼠肠缺血再灌注诱发肝损伤的影响及不同阿片受体在其中的作用.方法 成年健康雄性SD大鼠54只,体重200～300 g,采用随机数字表法分为9组（n=6）,假手术组（S组）仅分离肠系膜上动脉,不夹闭;缺血再灌注组（I/R组）、羟考酮预处理组（OP组）、μ受体拮抗剂CTOP组（CTOP组）、δ受体拮抗剂纳曲吲哚组（NTD组）、κ受体拮抗剂nor-binaltorphimne组（BNI组）、CTOP＋羟考酮预处理组（CTOP＋OP组）、纳曲吲哚＋羟考酮预处理组（NTD＋OP组）和nor-binaltorphimne＋羟考酮预处理组（BNI＋OP组）采用夹闭肠系膜上动脉45 min,再灌注2h的方法制备肠缺血再灌注损伤模型;OP组、COTP＋OP组、NTD＋OP组和BNI＋OP组于缺血前10 min时静脉注射羟考酮0.5 mg/kg;COTP＋OP组和NTD＋OP组于缺血前20 min时分别静脉注射COTP 1mg/kg或纳曲吲哚5 mg/kg,BNI＋OP组于缺血前25 min时静脉注射nor-binaltorphimne 5 mg/kg;CTOP组和NTD组分别于缺血前10 min时静脉注射相应剂量CTOP和纳曲吲哚;BNI组于缺血前15 min时静脉注射相应剂量nor-binaltorphimne.于再灌注2h时处死大鼠,取肝组织,光镜下观察病理学结果,采用TUNEL染色法观察肝细胞凋亡情况,计算细胞凋亡指数.结果 与S组比较,I/R组、OP组、CTOP组、NTD组、BNI组、COTP＋OP组、NTD＋OP组和BNI＋OP细胞凋亡指数升高（P＜0.05）;与I/R组比较,OP组、COTP＋OP组、NTD＋OP组和BNI＋OP组细胞凋亡指数降低（P＜0.05）,肝病理学损伤减轻,CTOP组、NTD组和BNI组细胞凋亡指数差异无统计学意义（P＞0.05）,肝病理学损伤无差异;与OP组比较,COTP＋OP组、NTD＋OP组和BNI＋OP组细胞凋亡指数升高（P＜0.05）,肝病理学损伤加重;COTP＋OP组、NTD＋OP组和BNI＋OP组间细胞凋亡指数比较差异无统计学意义（P＞0.05）,肝病理学损伤无差异.结论 羟考酮预处理可减轻大鼠肠缺血再灌注诱发肝损伤,μ、δ和κ受体Objective To evaluate the effect of oxycodone preconditioning on liver injury induced by intestinal ischemia-reperfusion （I/R） in rats and the role of different opioid receptors.Methods Fiftyfour adult male Sprague-Dawley rats, weighing 200-300 g, were randomly divided into 9 groups （n =6 each） using a random number table： sham operation group （group S）, group I/R, oxycodone preconditioning group （group OP） , μ receptor antagonist CTOP group （group CTOP） , δ receptor antagonist naltrindole group （group NTD）, κ receptor antagonist nor-binaltorphimne group （group BNI）, CTOP ＋ oxycodo ne preconditioning group （group CTOP＋OP） , naltrindole ＋ oxycodone preconditioning group （group NTD＋ OP） , and nor-binaltorphimne ＋ oxycodone preconditioning group （BNI＋OP）.The model of intestinal I/R was established by occlusion of the superior mesenteric artery for 45 min followed by 2 h reperfusion in anesthetized rats.The superior mesenteric artery was only exposed, but not occluded in group S.In OP,COTP＋OP, NTD＋OP and BNI＋OP groups, oxycodone 0.5 mg/kg was injected intravenously at 10 min prior to ischemia.COTP 1 mg/kg and naltrindole 5 mg/kg were injected intravenously at 20 min prior to ischemia in COTP＋OP and NTD＋OP groups, respectively.Nor-binaltorphimne 5 mg/kg was injected intravenously at 25 min prior to ischemia in group BNI＋OP.In CTOP and NTD groups, the corresponding doses of CTOP and naltrindole were injected intravenously at 10 min prior to ischemia.In group BNI, the corresponding dose of nor-binaltorphimne was injected intravenously at 15 min prior to ischemia.The rats were sacrificed at 2 h of reperfusion, and left hepatic lobes were removed for microscopic examination and for detection of apoptosis in liver cells （using TUNEL）.The apoptosis index （AI） was calculated.Results Compared with group S, the AI was significantly increased in the other groups （P〈0.05）.Compared with group I/R, the AI was significantly decreased （P〈0.05） ,

关 键 词：羟可酮 缺血预处理 再灌注损伤 肠 受体 阿片样 肝损伤 

分 类 号：R614[医药卫生—麻醉学]