JNK信号通路在紫杉醇诱发大鼠海马神经元凋亡中的作用：与NF-κB通路的关系  

Role of JNK signaling pathway in paclitaxel-induced apoptosis in hippocampal neurons of rats： the relationship with NF-κB pathway

作　　者：吴川 柴潇潇 王秀丽 赵爽 金子 李昭 郭跃先 

机构地区：[1]河北医大学第三医院麻醉科,石家庄市050051 [2]河北医大学第三医院泌尿外科,石家庄市050051

出　　处：《中华麻醉学杂志》2015年第11期1347-1349,共3页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（30772082）

摘　　要：目的 评价c-Jun氨基末端激酶（JNK）信号通路在紫杉醇诱发大鼠海马神经元凋亡中的作用及其与NF-κB通路的关系.方法 培养大鼠原代海马神经元,以1＆#215;106个/ml密度接种于96孔培养板,200 μl/孔,32个培养孔,采用随机数字表法,将其分为4组（n=8）：正常对照组（C组）、紫杉醇组（P组）、JNK抑制剂SP600125组（S组）和SP600125＋紫杉醇组（S＋P组）.P组加入2 ml紫杉醇（1 μmol/L）;S组加入2 ml SP600125（10 μmol/L）;S＋P组先加入2 ml SP600125（10μ ,mol/L）,孵育1h时加入2 ml紫杉醇（1μmol/L）.紫杉醇孵育24 h时,采用流式细胞术测定海马神经元凋亡率,采用Western blot法测定NF-κB p65表达.结果 与C组比较,P组和S＋P组神经元凋亡率升高,NF-κB p65表达上调,S组神经元凋亡率降低,NF-κB p65表达下调（P＜0.05）;与P组比较,S＋P组神经元凋亡率降低,NF-κB p65表达下调（P＜0.05）.结论 JNK信号通路介导了紫杉醇诱发大鼠神经元凋亡的过程,其机制可能与抑制NF-κB通路激活有关.Objective To evaluate the role of c-Jun N-terminal kinase （JNK） signaling pathway in paclitaxel-induced apoptosis in hippocampal neurons of rats, and the relationship with nuclear factor kappa B （NF-κB） pathway.Methods The primarily cultured hippocampal neurons were seeded in 96-well plate at a density of 1＆#215;106 cells/ml （200 μl/hole） , and were randomly divided into 4 groups （n=8 each） using a random number table： control group （C group）, paclitaxel group （P group）, JNK inhibitor SP600125 group （S group）, and SP600125 ＋ paclitaxel group （S＋P group）.Paclitaxel 2 ml （1 μmol/L） was added to group P.SP600125 2 ml （10 μmol/L） was added to group S.In group S＋P, SP600125 2 ml （10 μmol/L） was added, the cell were then incubated for 1 h, and then paclitaxel 2 ml （1 μmol/L） was added.The cells were then incubated for 24 h.At 24 h of incubation, the apoptosis in hippocampal neurons was detected by flow cytometry, and the expression of NF-κB p65 was measured by Western blot.The apoptosis rate was calculated.Results Compared with group C, the apoptosis rate was significantly increased, and the expression of NF-κB p65 was up-regulated in P and S＋P groups, and the apoptosis rate was significantly decreased, and the expression of NF-κB p65 was down-regulated in group S （P〈0.05）.Compared with group P, the apoptosis rate was significantly decreased, and the expression of NF-κB p65 was down-regulated in group S＋P （P〈0.05）.Conclusion JNK signaling pathway mediates paclitaxel-induced apoptosis in hippocampal neurons of rats, and the mechanism is likely related to inhibition of NF-κB pathway activation.

关 键 词：紫杉酚 神经元 海马 细胞凋亡 JNK丝裂原活化蛋白激酶类 

分 类 号：R614[医药卫生—麻醉学]