异丙酚和七氟醚对阿霉素化疗诱发大鼠心肌损伤的影响  

Effects of propofol and sevoflurane on adriamycin chemotherapy-induced myocardial damage in rats

作　　者：赵洪伟[1] 郭东勇[1] 杨泉涌[1] 李锦成[1] 

机构地区：[1]天津医科大学肿瘤医院麻醉科 国家肿瘤临床医学研究中心 天津市肿瘤防治重点实验室,300060

出　　处：《中华麻醉学杂志》2015年第11期1362-1364,共3页Chinese Journal of Anesthesiology

基　　金：天津市卫生局科技基金（2011KZ76）

摘　　要：目的 探讨异丙酚和七氟醚对阿霉素化疗诱发大鼠心肌损伤的影响.方法 健康雄性Wistar大鼠24只,3月龄,体重212～270 g,采用随机数字表法,分为4组（n=6）,对照组（C组）腹腔注射等容量的生理盐水;阿霉素组（ADM组）腹腔注射阿霉素2.5 mg/kg,隔日1次,共6次（共11d）;阿霉素-异丙酚组（ADM-Pro组）阿霉素给药方法同ADM组,最后一次给药后3d,实施异丙酚麻醉：腹腔注射异丙酚总量200 mg/kg,首次100 mg/kg,1h后追加剩余量,麻醉时间约2h;阿霉素-七氟醚组（ADM-Sevo组）阿霉素给药方法同ADM组,最后一次给药后3d,实施七氟醚麻醉：吸入1.5％ ～3.0％七氟醚,维持2h.麻醉结束后,取血标本,采用免疫发光技术测定血清cTnI浓度.取心肌组织,采用免疫组化法测定心肌组织caspase-3的表达.结果 与C组比较,ADM组、ADM-Pro组和ADM-Sevo组血清cTnI浓度升高,心肌组织caspase-3表达上调（P＜0.05）;与ADM组比较,ADM-Pro组和ADM-Sevo组血清cTnI浓度降低,心肌组织caspase-3表达下调（P＜0.05）;ADM-Pro组和ADM-Sevo组上述指标比较差异无统计学意义（P＞0.05）.结论 异丙酚和七氟醚麻醉均可减轻阿霉素化疗诱发的大鼠心肌损伤,效果无明显差异.Objective To investigate the effects of propofol and sevoflurane on adriamycin chemotherapy-induced myocardial damage in rats.Methods Twenty-four healthy male Wistar rats, aged 3 months, weighing 212-270 g, were randomly divided into 4 groups （n =6 each） using a random number table： control group （group C） , adriamycin group （group ADM） , adriamycin-propofol group （group ADM-Pro） , and adriamycin-sevoflurane group （group ADM-Sevo）.The equal volume of normal saline was injected intraperitoneally in group C.In group ADM, adriamycin 2.5 mg/kg was injected intraperitoneally once every other day for 6 times （11 days in total）.In group ADM-Pro, adriamycin 2.5 mg/kg was injected intraperitoneally once every other day for 6 times, and at 3 days after the last administration of adriamycin, propofol anesthesia was performed as follows ： the total amount of propofol injected intraperitoneally was 200 mg/kg, the initial dose was 100 mg/kg, and 1 h later the remaining amount was added, and the duration of anesthesia was about 2 h.In group ADM-Sevo, adriamycin 2.5 mg/kg was injected intraperitoneally once every other day for 6 times, and at 3 days after the last administration of adriamycin, sevoflurane anesthesia was performed as follows： 1.5%-3.0% sevoflurane was inhaled for 2 h.After the end of anesthesia, blood samples were collected for determination of serum cardiac troponin （cTnI） concentrations.Myocardial specimens were collected for detection of caspase-3 expression by immuno-histochemistry.Results Compared with group C, the serum cTnI concentration was significantly increased, and the caspase-3 expression was up-regulated in ADM, ADM-Pro and ADM-Sevo groups （P〈 0.05）.Compared with group ADM, the serum cTnI concentration was significantly decreased, and the caspase-3 expression was down-regulated in ADM-Pro and ADM-Sevo groups （P〈0.05）.There was no significant difference in the parameters mentioned above between group ADM-Pro and group ADM-Sevo （P〉0.05）.Conclusion Pr

关 键 词：多柔比星 二异丙酚 麻醉药 吸入 药物毒性 

分 类 号：R614[医药卫生—麻醉学]