七氟醚对失血性休克猪脑损伤的影响  

Effect of sevoflurane on brain injury in pigs with hemorrhagic shock

作　　者：王洪乾[1] 陈克研[1] 张铁铮[1] 陈万成[1] 

机构地区：[1]沈阳军区总医院麻醉科,110016

出　　处：《中华麻醉学杂志》2015年第11期1395-1397,共3页Chinese Journal of Anesthesiology

基　　金：全军“十二五”医学科研项目（BWSl2J008）

摘　　要：目的 评价七氟醚对失血性休克猪脑损伤的影响.方法 成年雄性巴马小型猪24头,6月龄,体重22～25 kg,采用随机数字表法分为3组（n=8）：假手术组（Sham组）不放血,仅穿刺双侧股动脉、颈内静脉;失血性休克组（HS组）按容量控制性放血制备失血性休克猪脑损伤模型;七氟醚组（S组）制备模型成功后吸入2％七氟醚30 min.于造模前即刻（T0）、失血性休克后30、60、90、120、180和240 min（T1-6）时采集颈内静脉血样,采用ELISA法测定血清IL-1β和TNF-α的浓度,采用双抗体夹心法测定血清神经元特异性烯醇化酶（NSE）和S-100β蛋白的浓度.结果 与Sham组比较,HS组和S组T2-6时血清IL-1β、TNF-α、NSE和S-100β蛋白的浓度升高（P＜0.05）;与HS组比较,SP组T3-6时血清IL-1β、TNF-α、NSE和S-100β蛋白的浓度降低（P＜0.05）.结论 七氟醚可减轻失血性休克猪脑损伤,其机制与抑制炎性反应有关.Objective To evaluate the effect of sevoflurane on brain injury in pigs with hemorrhagic shock （HS）.Methods Twenty-four adult male Bama miniature pigs, aged 6 months, weighing 22-25 kg, were equally and randomly divided into 3 groups using a random number table： sham operation group （group Sham） , group HS, and sevoflurane group （S group）.In group Sham, the bilateral femoral arteries and internal jugular vein were only punctured.The animals were anesthetized with iv propofol 3.0 mg/kg, tracheostomized and mechanically ventilated.The right femoral artery was cannulated for blood-letting.HS was induced by blood-letting （40% blood volume within 15 min）, and it was then maintained for 1 h after the end of blood-letting to induce brain injury.In group S, 2% sevoflurane was inhaled for 30 min after successful establishment of the model.Immediately before establishment of the model （T0） , and at 30, 60,90, 120, 180 and 240 min after HS （T1-6） , blood samples were collected from the internal jugular vein for determination of interleukin-1beta （IL-1β） and tumor necrosis factor-alpha （TNF-α） concentrations in serum （by enzyme-linked immunosorbent assay）, and neuron-specific enolase （NSE） and S-100β protein concentrations in serum （using double antibody sandwich method）.Results Compared with group Sham, the serum IL-1β, TNF-α, NSE and S-100β protein concentrations were significantly increased at T2-6 in HS and S groups （P〈0.05）.Compared with group HS, the serum IL-1β, TNF-α, NSE and S-100β protein concentrations were significantly decreased at T3-6 in group S （P〈 0.05）.Conclusion Sevoflurane can mitigate brain injury in pigs with HS, and the mechanism is associated with inhibition of inflammatory responses.

关 键 词：麻醉药 吸入 休克 出血性 脑损伤 

分 类 号：R614[医药卫生—麻醉学]