心跳骤停复苏大鼠海马冷诱导RNA结合蛋白表达的变化  

Changes in expression of cold-inducible RNA binding protein in hippocampus after cardiopulmonary resuscitation in a rat model of cardiac arrest

作　　者：吴淋 徐苗苗[2] 高宇[2] 仲浩[1] 惠康丽[2] 夏明[2] 段满林[2] 徐建国[2] 

机构地区：[1]徐州医学院江苏省麻醉学重点实验室,221004 [2]南京大学医学院南京军区南京总医院麻醉科

出　　处：《中华麻醉学杂志》2015年第11期1398-1401,共4页Chinese Journal of Anesthesiology

基　　金：天普研究基金（UF201407）

摘　　要：目的 评价心跳骤停复苏大鼠海马冷诱导RNA结合蛋白（CIRP）表达的变化.方法 健康雄性SD大鼠85只,体重280～ 320 g,3月龄,采用随机数字表法,将其分为3组：正常对照组（C组,n=15）、假手术组（S组,n=15）和心跳骤停复苏组（CPR组,n=55）.CPR组采用经食管心脏起搏诱发室颤的方法制备心跳骤停模型,随后进行复苏,分别于复苏后6h、12h、1d、3d和7d时取海马组织,采用RT-PCR法检测CIRP mRNA表达,采用Western blot法检测CIRP表达.复苏后3d取脑组织,采用组织化学染色法检测海马CA1区CIRP表达.结果 与C组比较,CPR组复苏后1、3、7d时海马CIRP mRNA表达上调,复苏后1、3d时海马CIRP表达上调（P＜0.05）,S组上述指标差异无统计学意义（P＞0.05）;与复苏后3d时比较,CPR组其它时点海马CIRP mRNA表达下调,复苏后6h、12h、7d时海马CIRP表达下调（P＜0.05）.CIRP主要表达于海马CA1区锥体细胞的细胞核.结论 心跳骤停复苏大鼠海马CIRP表达上调是抑制脑组织细胞凋亡的内源性机制之一.Objective To evaluate the changes in the expression of cold-inducible RNA binding protein （CIRP） in the hippocampus after cardiopulmonary resuscitation （CPR） in a rat model of cardiac arrest.Methods Eighty-five male Sprague-Dawley rats, weighing 280-320 g, aged 3 months, were randomly divided into 3 groups using a random number table： control （group C, n =15） , sham operation group （group S, n=15） and CPR group （n=55）.Cardiac arrest was induced through ventricular fibrillation caused by transoesophageal cardiac pacing, and then CPR was performed in group CPR.At 6 h, 12 h, and 1, 3 and 7 days after CPR, and the hippocampi were removed for detection of the expression of CIRP mRNA （using real-time reverse transcriptase polymerase chain reaction） and CIRP protein （using Western blot）.The brain tissues were also obtained at 3 days after CPR for detection of CIRP expression in the hippocampal CA1 region by immunohistochemistry.Results Compared with group C, the expression of CIRP mRNA was significantly up-regulated at 1, 3, and 7 days after CPR, and the expression of CIRP was up-regulated at 1 and 3 days after CPR in group CRP （P〈0.05） , and no significant change was found in the parameters mentioned above in group S （P〉0.05）.Compared with the values at 3 days after CPB, the expression of CIRP mRNA was significantly down-regulated at each time point after CPR, and the expression of CIRP was down-regulated at 6 h, 12 h, and 7 days after CPR in group CRP （P〈0.05）.CIRP was mainly expressed in the nucleus of pyramidal cells in the hippocampal CA1 region.Conclusion Upregulated expression of hippocampal CIRP is one of the endogenous mechanisms that inhibit cell apoptosis in the brain tissues of rats.

关 键 词：心脏停搏 人工 心肺复苏术 RNA结合蛋白质类 海马 

分 类 号：R541.78[医药卫生—心血管疾病]