CEUS定量分析L-NAME致兔高血压性肾损伤的实验研究  

Quantitative analysis of renal injury using CEUS in hypertensive rabbits model induced by L-NAME

作　　者：孟祥鹿[1,2] 张国慧[3] 王文红[2] 邵红[2] 郭君[3] 赵效新 顾程[1,2] 张晓丹[4] 

机构地区：[1]天津医科大学研究生院,300070 [2]天津市人民医院 [3]航天中心医院超声科 [4]河北大学附属医院超声科

出　　处：《天津医药》2015年第12期1394-1397,共4页Tianjin Medical Journal

基　　金：天津市卫生局基金资助项目(2011KZ52)

摘　　要：目的应用超声造影(CEUS)定量评价一氧化氮合成酶抑制剂N-硝基-L-精氨酸甲酯(L-NAME)致兔高血压肾损伤后肾皮质的血流灌注变化。方法建立L-NAME致兔高血压肾损伤模型,分别于给药前及给药后第2、4、6、8周行CEUS检查及血清胱抑素C(CysC)等生化检查,将所获得的时间-强度曲线(TIC)曲线下面积(AUC)等相关参数进行定量分析,并将AUC与血清CysC进行相关性分析。结果兔血清CysC在给药6周后明显升高,明显早于血肌酐(Scr)及血尿素氮(BUN)。给药后AUC先减小后增大,开始增强时间(RT)延长,峰值强度(PI)减低,平均通过时间(MTT)、峰值减半时间(HPT)及达峰时间(TTP)延长。CEUS所得AUC与血清CysC呈显著正相关(r=0.950,P<0.001)。结论 L-NAME致兔高血压肾损伤模型操作简单、可重复性好,可用于对高血压肾损伤临床前期及临床期的药物实验研究。CEUS结合血清CysC可作为临床评价高血压肾损伤患者肾功能异常的良好检查方法。Objective To assess the value of contrast-enhanced ultrasound （CEUS） on quantitative analysis of renal cortex perfusion in hypertensive rabbits model. Methods Hypertensive rabbit modal （n=10） were established by injecting N-nitro-L-arginin methylester （L-NAME）. CEUS and Cystatin C （CysC） serum level analysis were performed at different time points：before and the 2nd, 4th, 6th and 8th week after injecting L-NAME. Time-intensity curve and area under curve （AUC） were analyzed quantatively while correlation of AUC and CysC were also analyzed. Results Serum level of Cys C increased significantly at the 6th week after L-NAME administration which is earlier than the increase of serum levels of Scr and BUN. AUC decreased at first then increased after L-NAME administration. Upon addition of L-NAME, rise time （RT） and peak intensity （PI） decreased while mean transit time （MTT）, time from peak to one half （HPT） and time to peak （TTP） increased. Our study confirmed a positive correlation between AUC and Cys C （r=0.950, P〈0.001）. Conclusion Setting up rabbits model by L-NAME is convenient and reproducible, which is an useful tool in experimental study of preclinical and clinical phase of hypertensive renal injury. CEUS combining with CysC serum level analysis is considered as an effective technology for evaluating renal function in hypertensive patients.

关 键 词：高血压 NG-硝基精氨酸甲酯 兔 高血压肾损伤 超声造影 血清胱抑素C 肾血流灌注 

分 类 号：R445.1[医药卫生—影像医学与核医学]