功能性自组装纳米多肽水凝胶负载脂肪间充质干细胞的研究  

Functionalized self - assembling peptide hydrogel loaded with adipose derived mesenchymal stem cells

作　　者：刘占鳌[1] 黄文柏[1] 周冠洲 范鲁峰[1] 邵闻冲 胡三元[1] 孙念峰[1] 

机构地区：[1]山东大学齐鲁医院普外科,济南250012

出　　处：《中华实验外科杂志》2015年第12期3007-3009,共3页Chinese Journal of Experimental Surgery

基　　金：山东大学齐鲁医院（青岛）科研基金资助项目（QDKY2015ZD05）;山东省自然科学基金资助项目（ZR2012HM002）;中国博士后科学基金资助项目（20100481271）

摘　　要：目的观察功能性自组装多肽的理化性质、自组装成水凝胶特性及对细胞进行三维培养。方法合成RADA、RGD、KLT3种多肽并制备RAD／KLT!RGD多肽混合溶液，盐溶液诱发多肽溶液自组装成水凝胶。原代培养脂肪间充质干细胞并对其进行鉴定，使用水凝胶对其三维培养来观察其在水凝胶中的生长，并设置RAD／KLT水凝胶组做对比。结果多肽溶液成功自组装成水凝胶，干细胞在三维培养中迁移、分化并有成管腔的趋势，RAD／KLT／RGD水凝胶[（87．75±4．79）个细胞／视野]较对比组[（65．50±6．25）个细胞／视野]黏附生长了更多的细胞（P〈0．05）。结论RAD／KLT／RGD功能性自组装纳米多肽水凝胶是一种更为优良的组织工程框架材料。Objective To observe the properties of the functionalized self- assembling peptide hydrogel, and investigate the three - dimensional culture of the adipose derived mesenchymal stem cells （Ad- MSCs）. Methods Peptides （RADA, RGD and KLT） were prepared and functionalized self- as- sembling peptide solution （RAD/KLT/RGD） was prepared by mixing RADA, RGD and KLT solutions with a volume ratio of 2：1：1. Ad - MSCs were isolated, cultured and identified. After the peptide hydrogel was successfully formed via the self - assemble process, three - dimensional cell culture was performed. And RAD/KLT hydrogel was designed as the control group. Results After the peptide solution was in- duced by phosphate buffer （PBS）, hydrogel was constructed. Ad - MSCs stretched well and connected each other, inclined to format lumen structure when three - dimensional culture was done. RAD/KLT! RGD hydrogel [ （87. 75 ± 4. 79） cells per visual field ] contained more cells than control group [ （65.50±6. 25） cells per visual field, P 〈 0. 05 ]. Conclusion Functionalized self - assembling peptide hydrogel RAD/KLT/RGD can be a more suitable tissue engineering scaffold.

关 键 词：功能性自组装多肽 水凝胶 脂肪来源的间充质干细胞 组织工程 

分 类 号：R681[医药卫生—骨科学]