胰高血糖素样肽-1对原发性高血压大鼠心肌微血管损伤的保护作用  

Glucagon - like peptide - 1 on spontaneously hypertensive rats protective effects of cardiac micro- vascular injury research

作　　者：丁同斌[1] 法宪恩[2] 简立国[1] 刘畅[1] 赵江涛[1] 刘士超[1] 

机构地区：[1]郑州大学第二附属医院心内科,450014 [2]郑州大学第二附属医院心外科

出　　处：《中华实验外科杂志》2015年第12期3086-3088,共3页Chinese Journal of Experimental Surgery

摘　　要：目的观察胰高血糖素样肽-1（GLP-1）对原发性高血压大鼠心肌微血管损伤的保护作用。方法120只SHR大鼠随机平均分为4组，分别用于药效实验和细胞实验的对照组和实验组（30只）。药效试验：实验组皮下注射艾塞那肽（GLP-1类似物），每日1次，连续7d，对照组注射生理盐水，第8天测定并比较两组大鼠血压、心率、左心室厚度、微动脉密度、壁腔比值、毛细血管密度和内皮细胞面积的差异。细胞实验：提取实验组和对照组大鼠的心肌微血管内皮细胞（CMECs），消化后接种于6孔板，传2～3代，对照组常规细胞培养，研究组采用GLP-1预处理培养，超氧化物试刹盒检测活性氧（ROS）生成，原位缺口末端标记法（TUNEL）法检测CMECs凋亡。结果药效试验：交验组给药后血压（127．81±10．41）mmHg[1mmHg=0．133kPa，P〈0．05]、心率（363．1±15．9）次／分（P〈0．01）以及微动脉特征指标左心室厚度（10．71±1．20）mm（P〈0．05）、微动脉密度4．48±2．21（P〈0．05）、壁腔比值1．33±0．18（P〈0．05）和内皮细胞面积17．10±5．27（P〈0．01）较对照组均显著降低，但毛细血管密度显著升高159．7±50．1（P〈0．05）。细胞试验：实验组ROS荧光强度0．25，明显低于对照组（P〈0．05），CMECs凋亡率（39．30±3．12）％，明显高于对照组（χ2=10．67，P〈0．05）。结论GLP-1干预能够逆转原发性高血压大鼠左心室肥厚，改善其心脏功能，并降低SHR大鼠壁腔比值，增加毛细血管密度和病变细胞凋亡率，对原发性高血压患者心肌微血管损伤具有一定的保护作用。Objective Explore protective function of glucagon - like peptide - 1 （ GLP - 1 ） in es- sential hypertension patients with microvascular injury. Methods 120 SHR rats were divided into 4 groups, which were study groups and control groups of efficacy trials and cell trials. Efficacy trials：study Group＇s rats were injected subcutaneously exenatide （GLP-1 analogues） ,control Group＇s rats were injec- ted with saline, compared the blood pressure, heart rate, left ventrieular thickness, micro-arterial density, wall cavity ratio, capillary density, endothelial cell area. Cell trials：extracted microvascular endothelial cells （CMECs） of rat heart muscle from control group and study group, then CMECs were inoculated in 6-well plates after digested, cultured 2-3 generation, the control group received conventional cell culture, the study group was cultured with GLP-1 pretreatment. Detected the reactive oxygen species （ROS） fluorescence in- tensity and CMECs apoptosis. Results Efficacy trials ： study group＇ s blood pressure （ 127.81 ± lO. 41 ） mmHg [ 1 mmHg = 0. 133 kPa, P 〈 0. 05 ], heart rate （ 363.1± 15.9 ）/min （ P 〈 0. 01 ）, left ventricular thickness （10.71 ± 1.20） mm （P 〈0.05） ,micro-arterial density 4.48 ±2. 21 （P 〈0.05） ,wall cavity ra- tio 1.33 ± 0. 18 （ P 〈 0. 05 ） and endothelial cell area 17.10 ± 5.27 （ P 〈 0. 01 ） were all lower than control group,but capillary density were higher 159. 7 ± 50. 1 （P 〈 0. 05）. Cell test：study group＇ s ROS fluores- cence intensity 0. 25 were significantly lower than control group （P 〈 0. 05 ）. but CMECs apoptosis rate （39. 30 ± 3. 12） % were significantly higher control group （χ2 = 10. 67, P 〈 0. 05 ）. Conclusion GLP-1 can reverse left ventricular hypertrophy, improve heart function and reduce wall cavity ratio,increase capillary density and endothelial ceils numbers, which can protect myocardial microvascular injury in essential hypertension patients.

关 键 词：胰高血糖素样肽-1 原发性高血压 微血管损伤 

分 类 号：R587.1[医药卫生—内分泌]