微小RNA-27a靶向调控过氧化物酶体增殖子活化受体-γ和骨形态发生蛋白-2对激素诱导大鼠骨髓间充质干细胞分化的影响  

Effects on differentiation of steroid - induced bone marrow mesenchymal stem cells of rats by targe- ting regulation of microRNA - 27a on peroxisome proliferator activated receptor - γ/and bone mor- phogenetic protein- 2

作　　者：王光辉[1] 李月白[1] 李明[1] 谷晨熙[1] 宋石[2] 单杰[2] 赵国强[3] 王义生[1] 

机构地区：[1]郑州大学第一附属医院骨科河南省高等学校临床医学重点学科开放实验室,450052 [2]郑州大学基础医学院生物化学与分子生物学系 [3]郑州大学基础医学院微生物免疫系

出　　处：《中华实验外科杂志》2015年第12期3110-3112,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（81470106）

摘　　要：目的检测微小RNA（miRNA，miR）-27a调控过氧化物酶体增殖子活化受体-γ（PPAR-γ）和骨形态发生蛋白-2（BMP-2）对骨髓间充质干细胞（BMSCs）分化的影响。方法将培养的40只大鼠BMSCs，随机分4组，正常对照组：细胞不作特殊处理；模型组：细胞给予1×10-7mol／L地塞米松；无关序列组：将无关序列基因电转入细胞，给予1×10 -7mol／L地塞米松；实验组：将具有双向靶向作用的miR-27a电转入细胞，给予1×10-7mol／L地塞米松。采用实时荧光定量聚合酶链反应（RT—qPCR）技术测定PPAR-γ和BMP-2mRNA的相对表达量。结果处理细胞7d时，实验组细胞中PPAR-γmRNA的相对表达量（1．203±0．111）较模型组（1．877±0．225）、无关序列组（1．913±0．195）明显降低（P〈0．05），近似于正常组（1．000）且与其差异无统计学意义（P〉0．05）。实验组细胞中BMP-2mRNA的相对表达量（0．832±0．105）较模型组（0．455±0．051）、无关序列组（0．422±0．038）明显升高（P〈0．05），近似于正常组（1．000）且与其差异无统计学意义（P〉0．05）。结论miR-27a能够有效抑制PPAR-γ表达，维持BMP-2表达。Objective To explore the effect on the differentiation of steroid- induced bone marrow mesenchymal stem cells （BMSCs） of rats by tarteting regulation of microRNA （miRNA, miR） -27a on per- oxisome proliferator - activated receptor γ, （ PPAR -γ） and bone morphogenetic protein - 2 （ BMP - 2 ）. Methods BMSCs of 40 rats were expanded and randomly divided into 4 groups. In normal control group, the ceils were not treated. In model group, the cells were treated with 1 0133× 10 -7 mol/L dexamethasone. In irrelative sequence group, the cells were electroporated with the irrelative sequence that was ineffective at targeting the PPAR -7 gene, and treated with 1 x 10-7 mol/L dexamethasone. In experimental group, the ceils were electroporated with miR - 27a and treated with 1 ~ 10-7 mol/L dexamethasone. The real - time quantitative reverse transcription polymerase chain reaction （RT -qPCR） was used to detected the expression of PPAR - ~/ and BMP - 2 mRNA R^sults At 7th day after treatment, the expression level of PPAR - ~/ mRNA in experimental group （ 1. 203 ± 0. 111 ） was significantly lower than that in model group （ 1. 877 -± 0. 225） and irrelative sequence group （1. 913 ± 0. 195 ） （P 〈 0.05 ）, and was similar to that in normal control group （ P 〉 0.05 ）. The expression level of BMP - 2 mRNA in experimental group （0. 832 ± 0. 105 ） was significantly higher than that in model group （0. 455 ±± 0. 051 ） and irrelative sequence group （ 0. 422 ± 0. 038 ） （ P 〈 0. 05 ）, and was similar to that in normal control group （ P 〉 0. 05 ）. Conclusion The miR- 27a may inhibit the expression of PPAR -%, effectively and maintain the expression of BMP- 2.

关 键 词：股骨头坏死 过氧化物酶体增殖子活化受体-γ 骨形态发生蛋白一2 

分 类 号：R686.5[医药卫生—骨科学]