荷载紫杉醇介孔二氧化硅纳米粒的制备及体外性能  

Preparation and in vitro properties of paclitaxel-loaded mesoporous silica nanoparticle

作　　者：张雪[1] 苏志桂[1] 薛玲静[1] 张灿[1] 

机构地区：[1]中国药科大学新药研究中心江苏省代谢性疾病药物研究重点实验室,南京210009

出　　处：《中国药科大学学报》2015年第6期653-658,共6页Journal of China Pharmaceutical University

摘　　要：介孔二氧化硅纳米粒(mesoporous silica nanoparticle,MSN)作为药物载体已成为纳米给药系统研究的热点。以无序孔道的MSN为载体,以溶剂吸附法负载化疗药物紫杉醇(PTX),从而制备得到PTX@MSN。考察了PTX@MSN的理化性质、药物体外释放行为和体外抗肿瘤活性等特性。研究结果表明,PTX@MSN载药量为(23.76±1.14)%,在水性介质中分散良好,粒径约为250 nm,电位为-(8.01±1.81)mV。PTX@MSN具有药物缓释特性,24h后PTX累积释放率为(23.62±2.15)%。细胞毒性结果显示,空白MSN生物安全性良好,而PTX@MSN组对人肝癌HepG2细胞的杀伤作用较市售Taxol组强。本研究为MSN递送抗肿瘤药物提供一定的理论与应用基础。Mesoporous silica nanoparticle as drug carrier has become the new research focus in the field of nanodrug delivery system in recent years. In this study, paelitaxel-loaded msesoporous silica nanoparticle （ PTX @ MSN） was manufactured by the solvent adsorption. In vitro studies revealed that PTX@ MSN was well dispersed in aqueous medium with particle size of 250 nm, the potential of - （8.01 ± 1.81） mV and drug loading efficiency of （23.76 ± 1.14） %. PTX@ MSN showed the sustained-release characteristics with the cumulative FFX of release （ 23.62 ± 2. 15） % at 24 h. In additions, the cytotoxicity investigation indicated that blank MSNs were biocompati- ble while PTX@ MSN group showed improved in vitro anti-tumor activity against HepG2 cell when compared with Taxol group. In conclusion, MSN is a promising platform to build drug delivery systems for tumor therapy.

关 键 词：介孔二氧化硅纳米粒 紫杉醇 体外释放 抗肿瘤活性 

分 类 号：R943[医药卫生—药剂学]