短小舌根草苷的制备及其在大鼠体内代谢产物的UPLC-QTOF/MS鉴定  

Preparation of pumiloside and identification of its metabolites in rats by UPLCQTOF/MS

作　　者：陈家全[1] 王慧[1,2] 张志远[3] 翟小婷 殷蓉[1,2] 朱粉霞[2] 

机构地区：[1]中国药科大学分析化学教研室,南京210009 [2]江苏省中医药研究院国家中医药管理局中药口服释药系统重点研究室,南京210028 [3]海南医学院药学院,海口570102

出　　处：《中国药科大学学报》2015年第6期677-682,共6页Journal of China Pharmaceutical University

基　　金：国家自然科学基金资助项目(No.81203001;No.81160558)~~

摘　　要：通过AB-8大孔吸附树脂对胆木药材水提液进行吸附,用不同比例的乙醇水溶液对其梯度洗脱,通过分离纯化获得纯度较高的短小舌根草苷单体。用UPLC-QTOF/MS分析技术鉴定大鼠灌胃短小舌根草苷后,尿液、粪便以及胆汁中该成分可能的代谢产物。采用全扫描方式采集所有成分准确MS/MS数据,利用MetaboLynx软件分析处理,获得了纯度较高的短小舌根草苷单体并鉴定出了其在大鼠体内的4种代谢产物。In this paper, water extract of Nauclea officinalis was absorbed by AB-8 macroporous resin and subsequently gradient-eluted with alcoholic solution of different proportion to prepare pumiloside monomer of high purity. The metabolites in urine, feces and bile of rats with gavage administration were analyzed by UPLC-QTOF/ MS. Accurate MS/MS data of all components were collected with full scan mode, and analyzed by MetaboLynx software. Results showed that the monomer of high-purity pumiloside was prepared and four metabolites in rats were identified.

关 键 词：短小舌根草苷 制备 代谢产物 UPLC-QTOF/MS法 

分 类 号：R283.6[医药卫生—中药学] R285.5[医药卫生—中医学]