建中理劳汤含药血清对TGF-β1诱导的大鼠肾小球系膜细胞增殖及基质金属蛋白酶表达的影响  

Effects of Jian-Zhong-Li-Lao Decoction-mediated Serum on TGF-β1-induced Proliferation of HBZY-1 and Expression of Matrix Metalloproteinases

作　　者：沈会[1,2] 陶汉华[1] 张诏[1] 展照双[1] 朱炜楷 赵妍妍[2] 

机构地区：[1]山东中医药大学基础医学院,济南250355 [2]大连医科大学附属第一医院,大连116011

出　　处：《世界科学技术-中医药现代化》2015年第9期1843-1848,共6页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家自然科学基金委面上项目(81273635):基于<金匮>"虚劳建中"理论探讨建中理劳汤对慢性肾功能衰竭及TGF-β/smad通路的影响研究;负责人:陶汉华

摘　　要：目的:通过观察建中理劳汤含药血清对转移生长因子-β1(TGF-β1)诱导的大鼠肾小球系膜细胞(HBZY-1)增殖、基质金属蛋白酶及其抑制酶表达的影响,探讨建中理劳汤治疗慢性肾衰纤维化的机制。方法:体外培养HBZY-1细胞,制备理劳汤含药血清,实验分为空白对照组、TGF-β1诱导组、空白血清组、建中理劳汤低、高剂量组和尿毒清组,通过乳酸脱氢酶(LDH)检测方法观察含药血清对HBZY-1细胞的毒性作用;通过CCK8方法观察各组HBZY-1细胞形态和增殖的情况;通过ELISA方法检测各组HBZY-1细胞中基质金属蛋白酶MMP-2、MMP-9及其抑制酶TIMP-1、TIMP-2表达的情况。结果:建中理劳汤含药血清对HBZY-1细胞无毒性作用(P>0.05);与模型组相比,建中理劳汤可明显抑制TGF-β1诱导的HBZY-1细胞的增殖(P<0.05);建中理劳汤可明显增加MMP-2、MMP-9的表达(P<0.05),同时抑制TIMP-1、TIMP-2的表达(P<0.05)。结论:建中理劳汤含药血清可明显抑制TGF-β1诱导的大鼠肾小球系膜细胞的增殖,并通过影响基质金属蛋白酶及其抑制酶的表达改善慢性肾衰纤维化。This study was aimed to observe the effect of Jian-Zhong-Li-Lao（JZLL） decoction-mediated serum on the TGF-β1-induced proliferation of HBZY-1 cells, the expression of matrix metalloproteinases and its inhibitory enzyme, in order to investigate the mechanisms of JZLL decoction in treatment of the renal fibrosis of chronic renal failure. HBZY-1 cells were cultured in vitro. JZLL decoction-mediated serum was prepared. The experiment contained the blank control group, TGF-β1-induced group, control serum group, low-dose JZLL decoction group, high-dose JZLL decoction group, and the Niao-Du-Qing group. The cytotoxic effects of JZLL decoction-mediated serum on HBZY-1 cells were assessed by LDH assay. The morphology and proliferation of HBZY-1 cells were examined by CCK8 assay. The expression of matrix metalloproteinases（MMP-2, MMP-9） and its inhibitory enzymes（TIMP-1, TIMP-2） were examined by ELISA assay. The results showed that there was no cytotoxic effect of JZLL decoction-mediated serum on HBZY-1 cells（P〉0.05）. Compared with the model group, JZLL decoction can obviously inhibit TGF-β1-induced proliferation of HBZY-1 cells（P〈0.05）. JZLL decoction can obviously increase the expressions of MMP-2 and MMP-9（P〈0.05）, and inhibit the expressions of TIMP-1 and TIMP-2（P〈0.05）. It was concluded that JZLL decoction-mediated serum significantly inhibited TGF-β1-induced proliferation of HBZY-1 cells, relieved the renal fibrosis of chronic renal failure through affecting the expression of matrix metalloproteinases and its inhibitory enzymes.

关 键 词：建中理劳汤 大鼠系膜细胞 TGF-Β1 纤维化 基质金属蛋白酶 

分 类 号：R289[医药卫生—方剂学]