一株虎源H5N1亚型禽流感病毒的分离鉴定及生物学特性研究  

Biological characteristic of an H5N1 avian influenza virus isolated from tiger in Guangxi province in 2015

作　　者：崔艳芳[1] 王德丽[1] 刘丽娜[1] 赵璐[1] 刘丽玲[1] 田国彬[1] 曾显营[1] 李雁冰[1] 陈化兰[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/国家禽流感参考实验室,黑龙江哈尔滨150001

出　　处：《中国预防兽医学报》2015年第12期908-911,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：农业部公益性行业专项-边境地区动物疫病防控技术体系研究(201103008);农业部动物流感监测与防治专项

摘　　要：2015年2月,从广西省南宁市动物园发病并死亡的白虎组织样品内分离到一株H5N1亚型禽流感病毒(AIV),命名为A/tiger/Guangxi/1/2015(H5N1)(简称Tiger/GX/1/15)。本研究对该分离株的全基因序列进行测定,并对其致病性进行研究。基因组序列分析表明:该病毒的HA基因属于Clade 2.3.2.1分支,HA蛋白的裂解位点处具有多个连续碱性氨基酸(^(341)RRRKR^(345)),具备高致病性禽流感病毒(HPAIV)的典型分子特征。BLAST分析显示该分离株的各基因节段分别与H5N1和H9N2亚型AIV的相应基因节段具有较高的相似性,推测该分离株为一株重组病毒。该病毒在小鼠的肺和鼻甲骨中能够进行有效的复制,对鼠的半数致死量(MLD_(50))>5.52 logEID_(50),对小鼠具有中等致病性。以上结果表明:该分离株未经适应便具备感染小鼠并对小鼠具有较高致死的能力。An H5NI subtype avian influenza virus （AIV） A/tiger/Guangxi/1/2015（H5N1） （Tiger/GX/1/15） was isolated from tigers in Nanning, Guangxi province and subjected to genome sequencing and pathogenic test in BALB/c mice. The genome analysis of HA showed that the virus was belonged to Clade 2.3.2.1 with a polybasic motif 341RReKe345 at the HA cleavage site, which was the molecular characterization of highly pathogenic AIV （HPA1V）. The sequence alignment results revealed that the isolate was a natural reassortant AIV of H5N1 with H9N2 subtypes. Animal artificial infection experiments indicated that the virus was medium pathogenic to mice with MLDs0 of 5.52 log EIDso. This study also highlights the importance of continues surveillance of AIV in mammalian animals.

关 键 词：禽流感病毒 H5N1亚型 重组 进化分析 

分 类 号：S852.65[农业科学—基础兽医学]