植物DNA断裂修复基因对农杆菌T-DNA整合的作用  

The Role of Plant DNA Break Repair Genes in Agrobacterium T-DNA Integration

作　　者：崔海瑞[1] 朱斌[1] 宋悦[1] 赵海军[1] 汪庆 

机构地区：[1]浙江大学原子核农业科学研究所农业部核农学重点开放实验室,杭州310029 [2]无锡求是生物农业有限公司,无锡214105

出　　处：《中国细胞生物学学报》2015年第11期1593-1600,共8页Chinese Journal of Cell Biology

基　　金：国家自然科学基金(批准号:11175154);农业公益性行业科研专项(批准号:201103007)资助的课题~~

摘　　要：转基因植物在作物新品种培育和生物制药中已发挥了巨大作用。农杆菌介导的遗传转化是广泛用于基因组分析的强大工具,也是获得转基因植物的主导技术。农杆菌介导的基因转移是极其复杂的生物学过程,需要许多农杆菌和植物的遗传因子协同参与完成。经过20多年的研究,人们对T-DNA产生和转运的分子机制以及农杆菌与寄主植物的互作已有所了解。T-DNA整合是农杆菌介导转化过程中最为关键的一步,但对于其整合机制所知仍有限。越来越多的证据表明,寄主植物细胞的DNA断裂修复基因对农杆菌T-DNA整合具有重要作用。该文首先介绍T-DNA转移的大致过程,重点讨论DNA断裂损伤修复相关基因对T-DNA整合的作用,为通过DNA损伤修复基因的遗传操纵来提高农杆菌介导植物遗传转化的效率提供参考。Transgenic plants have already played a great role in the development of crop new variety and biopharming. Agrobacterium-mediated genetic transformation has been widely used as a powerful tool for genome analysis and the predominant technology for production of transgenic plants. The gene transfer mediated by Agrobacterium is a very complex biological process involving the synergetic effect of genetic factors of both the bacterium and the host plant cell. Over the past two decades, a great deal has been learned about the molecular mechanism by which Agrobacterium produces T-DNA and transports it into the host nucleus, as well as the Agrobacterium-host plant interactions. However, T-DNA integration, the most critical step of the transformation process, largely remains an enigma. More and more evidences suggest that the DNA break repair genes of the host cell plays an important role in Agrobacterium T-DNA integration. In this review, the general process of Agrobacterium T-DNA transfer was introduced firstly and then we discussed the role of plant DNA break repair genes in T-DNA integration, to provide some useful information for the improvement of Agrobacterium-mediated transformation efficiency in plant by the genetic manipulation of DNA damage repair genes.

关 键 词：农杆菌 植物遗传转化 T-DNA整合 DNA断裂修复基因 

分 类 号：Q943.2[生物学—植物学]