载姜黄素介孔二氧化硅纳米粒的制备及其体外抗肿瘤活性研究  

Preparation and anti-cancer activity in vitro of curcumin loaded mesoporous silica nanoparticle

作　　者：何黎黎[1] 顾健[1] 

机构地区：[1]西南民族大学民族医药研究院,四川成都610041

出　　处：《中国中药杂志》2015年第21期4189-4193,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81302729);中央高校基本科研业务费专项(2015NZYQN16)

摘　　要：制备载姜黄素(curumin,Cur)的介孔二氧化硅纳米粒(mesoporous silica nanoparticles,MSN),考察其体外释药行为和抗肿瘤活性。采用聚合法制备了MSN,并运用溶剂挥干法和浸渍离心法制备了载Cur的MSN(Cur-MSN),以包封率和载药量为指标优化了制备工艺。通过扫描电镜表征了Cur-MSN形态,测定了Cur-MSN粒径大小和电位。以0.2%SDS溶液为释放介质,研究了Cur-MSN的体外释放行为,并考察了Cur-MSN对He La细胞的杀伤作用。运用优化工艺制备的Cur-MSN圆整均一,具有典型的介孔结构特征,平均粒径为75.8 nm,Zeta电位为-30.1 m V;3批Cur-MSN的平均包封率为(72.55±2.01)%,载药量为(16.21±1.12)%。Cur-MSN体外释药行为明显了缓释特征,96 h释放了83.5%;Cur-MSN对He La细胞的杀伤作用呈现浓度依赖性,IC50为19.40 mg·L-1,与Cur的杀伤作用相似。This paper is to prepare curcumin（ Cur） loaded mesoporous silica nanoparticle（ Cur-MSN）,evaluate its release behavior and anti-cancer activity in vitro. Mesoporous silica nanoparticle（ MSN） was prepared by polymerization method and Cur-MSN was obtained using solvent evaporation method and impregnation centrifugation method. The preparation method was optimized using entrapment efficiency（ EE） and loading efficiency（ LE） as indexes. Cur-MSN was characterized with scanning electron microscope and its particle size and zeta potential were determined. Finally,in vitro release behavior in 0. 2% SDS solution and its cell-killing effect on He La cells were also evaluated. The Cur-MSN prepared with process optimization method was round and uniform and exhibited typical mesoporous characterization. The mean particle size and Zeta potential of Cur-MSN were 75. 8 nm and- 30. 1 m V,respectively. EE and LE of three batches of Cur-MSN were（ 72. 55 ± 2. 01） % and（ 16. 21 ± 1. 12） %,respectively. In vitro release behavior of CurMSN showed a sustained release profile with 83. 5% cumulative release within 96 h. The killing effect of Cur-MSN on He La cells was dose-dependent with IC50 of 19. 40 mg·L- 1,which was similar to that of Cur.

关 键 词：姜黄素 介孔二氧化硅纳米粒 体外释药 抗肿瘤活性 

分 类 号：R283.6[医药卫生—中药学] R285[医药卫生—中医学]