短刺小克银汉霉对甘草次酸的微生物转化  

Microbial transformation of glycyrrhetinic acid by Cunninghamella blakesleeana

作　　者：马媛[1,2] 谢丹[3] 王照华[4] 戴均贵[3] 安熙强 顾政一 

机构地区：[1]新疆医科大学药学院,新疆乌鲁木齐830011 [2]新疆维吾尔自治区药物研究所,新疆乌鲁木齐830004 [3]中国医学科学院北京协和医学院药物研究所天然药物活性物质与功能国家重点实验室,北京100050 [4]河北出入境检验检疫局检验检疫技术中心,河北石家庄050051

出　　处：《中国中药杂志》2015年第21期4212-4217,共6页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81273405)

摘　　要：利用丝状真菌短刺小克银汉霉Cunninghamella blakesleeana CGMCC 3.970对甘草次酸(GA)进行微生物转化研究。甘草次酸与短刺小克银汉霉培养物在25℃,135 r·min-1条件下共孵育7 d后,采用溶剂萃取法、大孔吸附树脂硅胶柱色谱、半制备液相色谱等手段提取并分离纯化转化产物,获得1个主产物和5个次要产物,经MS、1H-NMR和13C-NMR分别鉴定为3-酮基-15α-羟基-18β-甘草次酸(1)、3-酮基-15β-羟基-18β-甘草次酸(2)、7β,15α-二羟基-18β-甘草次酸(3)、3-酮基-7β,15α-二羟基-18β-甘草次酸(4)、7β-羟基-18β-甘草次酸(5)和15α-羟基-18β-甘草次酸(6),其中化合物2为一新化合物。这些结果提示短刺小克银汉霉CGMCC 3.970对GA具有选择性酮基化及羟基化作用。A study on the microbial transformation of glycyrrhetinic acid（ GA） was conducted by a fungus,Cunninghamella blakesleeana CGMCC 3. 970 systematically. After incubation with the cell cultures of C. blakesleeana CGMCC 3. 970 at 25 ℃ for 7days on a rotary shaker operating at 135 r·min- 1,GA was converted into one major product and five minor products. The products were extracted and purified by solvent extraction,macroporous adsorbent resin,silica gel column chromatography,and semi-preparative RP-HPLC chromatography. Their structures were identified as 3-oxo-15α-hydroxy-18β-glycyrrhetinic acid（ 1）,3-oxo-15β-hydroxy-18β-glycyrrhetinic acid（ 2）,7β,15α-dihydroxy-18β-glycyrrhetinic acid（ 3）,3-oxo-7β,15α-dihydroxy-18β-glycyrrhetinic acid（ 4）,7β-hydroxy-18β-glycyrrhetinic acid（ 5） and 15α-hydroxy-18β-glycyrrhetinic acid（ 6） by the analyses of MS,1H-NMR and13C-NMR spectroscopic data respectively. Among them,2 was a new compound. These results suggest that C. blakesleeana CGMCC 3. 970 has the capability of selective ketonization and hydroxylation for GA.

关 键 词：甘草次酸 短刺小克银汉霉CGMCC 3.970 微生物转化 

分 类 号：R284[医药卫生—中药学]