黄芪通过ACE2/Mas途径改善代谢综合征大鼠早期肾功能损害  

Effect of Astragali Radix in improving early renal damage in metabolic syndrome rats through ACE2 / Mas pathway

作　　者：王琼英[1] 梁伟[1] 姜程[1] 李宁荫[1] 许涵[1] 杨米娜[1] 林欣[1] 于亨[1] 常鹏[1] 余静[1] 

机构地区：[1]兰州大学第二医院心内科,甘肃兰州730030

出　　处：《中国中药杂志》2015年第21期4245-4250,共6页China Journal of Chinese Materia Medica

基　　金：甘肃省中医药管理局重点课题(GZK-2010-Z1);甘肃省技术研究与开发专项计划项目(1205TCYA042);兰州大学第二医院院内中医药课题(YJzy2013-05)

摘　　要：探讨黄芪影响代谢综合征(MS)大鼠肾脏血管紧张素转换酶2(ACE2)以及血管紧张素1-7(Ang 1-7)受体Mas蛋白的表达及其抗氧化作用。将80只雄性SD大鼠分为:正常对照组、MS组、黄芪组、缬沙坦组。MS大鼠予以高脂肪高盐饲料,饮用10%果糖水制备代谢综合征动物模型,黄芪组予以黄芪6.0 g·kg-1·d-1灌胃,缬沙坦组予以缬沙坦30.0 mg·kg-1·d-1灌胃,MS组以及对照组予以同体积生理盐水灌胃,干预4周后行一般指标、生化指标以及血压测定,放免法检测血及肾组织血管紧张素Ⅱ(AngⅡ)、丙二醛(MDA)、一氧化氮(NO)和超氧化物歧化酶(SOD)水平,免疫蛋白印迹法检测各组大鼠肾组织Mas受体,ACE,ACE2,AT1R的表达。结果显示,与对照组比较,MS组、黄芪组大鼠收缩压、舒张压、体质量、空腹血糖、空腹胰岛素水平、甘油三酯、血清游离脂肪酸以及血浆、肾组织AngⅡ水平明显升高(P<0.05);缬沙坦组大鼠收缩压、舒张压与MS组比较明显降低;MS组大鼠肾组织NO含量以及SOD活性下降,经黄芪干预后较MS明显增高;黄芪治疗后较MS组大鼠肾脏组织中MDA水平明显降低(P<0.01)。与对照组比较,MS组肾组织中ACE和AT1R的表达明显升高,而ACE2和Mas受体的表达降低(均P<0.05);与MS组比较,MS+黄芪组肾组织中Mas受体的表达升高较显著,而MS+缬沙坦组AT1R的表达降低更明显(均P<0.05)。结论:黄芪可以提高肾脏组织Mas的表达,降低ACE表达,改变肾脏局部的AngⅡ,MDA,NO和SOD水平,从而对早期受损伤的肾组织起保护作用。To study the expression of angiotensin converting enzyme 2（ ACE2） and angiotensin（ Ang） 1-7 specific receptor Mas protain in renal blood vessels of metabolic syndrome（ MS） rats and its anti-oxidative effect. A total of 80 male SD rats were divided into four groups： the normal control group（ NC,the same volume of normal saline）,the MS group（ high fat diet）,the MS ＋ Astragali Radix group（ MS ＋ HQ,6 g·kg- 1·d- 1in gavage） and the MS ＋ Valsartan group（ MS ＋ XST,30 mg·kg- 1·d- 1in gavage）. After four weeks of intervention,their general indexes,biochemical indexes and blood pressure were measured; plasma and renal tissue AngⅡ,malondialdehyde（ MDA） and superoxide demutase（ SOD） levels were measured with radioimmunoassay. The protein expressions of Mas receptor,AT1 R,ACE and ACE2 were detected by western blot analysis. According to the result,compared with the NC group,the MS group and the MS ＋ HQ group showed significant increases in systolic and diastolic pressures,body weight,fasting glucose,fasting insulin,triglycerides,free fatty acid and AngⅡ level of MS rats（ P〈0. 05）. The MS ＋ XST group showed notable decreases in systolic and diastolic pressures than that of the MS group. The MS group showed significant increases in the SOD activity and NO level and decrease in the MDA level after being intervened with Astragali Radix. ACE and AT1 R protein expressions in renal tissues of the MS group were higher than that in the NC group,but with lower ACE2 and Mas receptor expressions（ all P〈0. 05）. Compared with the MS group,the MS ＋ HQ group showed significant increase in Mas receptor expression in renal tissues,whereas the MS ＋ XST group showed notable decrease in AT1R（ all P〈0. 05）. In conclusion,Astragali Radix can increase the Mas receptor expressions in renal tissues,decrease ACE expression and change local AngⅡ,MDA,NO and SOD in kidneys,so as to protect early damages in renal tissues.

关 键 词：黄芪 代谢综合征 氧化应激 MAS受体 

分 类 号：R285.5[医药卫生—中药学]