杨树烂皮病内生拮抗菌的筛选及鉴定  

Screening of antagonistic bacteria of Valsa sordida and identification of the strain NS3

作　　者：陈越渠[1,2] 李立梅[1] 毛赫 张立民[1] 王牧原[3] 李殿锋 张晓军[1] 

机构地区：[1]吉林省林业科学研究院,长春130033 [2]中国林业科学研究院森林生态环境与保护研究所,北京100091 [3]吉林省林业调查规划院,长春130022 [4]吉林省双辽市林业局森防站,吉林136400

出　　处：《植物保护》2015年第6期126-131,共6页Plant Protection

基　　金：林业公益性行业科研专项(201204501);吉林省科技发展计划青年基金(201201092);吉林省科技发展计划重点项目(20110267)

摘　　要：从健康杨树枝条上分离到1株能有效抑制杨树烂皮病菌的内生细菌,编号为NS3。该菌株对供试的13种植物病原菌均有抑制作用,抑菌谱广,其中对杨树烂皮病和杨树溃疡病病原菌抑制作用最强,其活菌的平均抑菌带宽度达到25.6mm以上,发酵液的抑菌圈直径达到48.6mm以上;人工接种防治试验结果表明:发酵液的无菌滤液对杨树烂皮病具有良好的生防作用,保护作用和治疗作用分别达85.4%和69.6%,与生产上用于防治该病的常用药剂防治效果差异不显著;根据其菌株形态、生理生化特征和16SrDNA序列比对,最终将该菌株鉴定为枯草芽胞杆菌(Bacillus subtilis)。An antagonistic bacterial strain NS3 against Vasal sordida was isolated from poplar branches.The NS3 strain displayed a broad-spectrum inhibition to all the 13 pathogenic fungi tested in this study.Of them,its inhibi-tive effect to Vasal sordida and Botryosphaeria dothidea was the strongest,with a width of inhibition zone of more than 25.6 mm and a diameter of 48.6 mm averagely for NS3 fermented broth.Meanwhile,the inoculation test suggested its great potential for biocontrol.The protection and curation of the fermented broth against V .sordida were raised to 85.4% and 69.6%,respectively.On the basis of the morphological and physiological-biochemical characteristics as well as 1 6S rDNA sequence analysis,the bacterium was identified as Bacillus subtilis .

关 键 词：枯草芽胞杆菌 分类鉴定 生防作用 

分 类 号：S763.1[农业科学—森林保护学] S718.8[农业科学—林学]