miRNA在植物病毒基因组中的靶基因预测及分析  

Prediction and analysis of target genes of plant miRNAs in virus genomes

作　　者：王艳芳[1] 赵彦宏[2] 于佳丽[1] 刘林德[1] 

机构地区：[1]鲁东大学生命科学学院,烟台264025 [2]鲁东大学农学院,烟台264025

出　　处：《植物保护》2015年第6期132-135,共4页Plant Protection

基　　金：国家自然科学基金项目(31201199);山东省自然科学基金项目(ZR2013CL013);水稻生物学国家重点实验室开放项目(120201)

摘　　要：microRNA(miRNA)是一类长约22nt的内源性的非编码小RNA,在植物的生长发育和胁迫响应中起重要调控作用。本文通过生物信息学的方法,在miRBase中下载了12种植物的miRNA序列;在DPVweb和VIDE病毒数据库中查找侵染这些植物的病毒,并在NCBI数据库中搜索其基因组序列,共获得173种病毒的基因组序列。用psRNATarget在线软件预测植物miRNA在植物病毒中的靶基因,发现植物miRNA可能参与调控包括聚合蛋白、衣壳蛋白、逆转录酶、复制相关基因、通读蛋白、依赖RNA的RNA聚合酶等多种植物病毒基因的表达。miRNAs are a group of non-coding small RNAs and play important roles in regulating the development and stress responses of plants.Sequences of miRNAs from 12 plants were downloaded from miRBase.Viruses in-vaded these plants were searched in DPVweb and VIDE.The sequences of these viruses were searched in NCBI. There were totally 1 73 sequences of viruses collected.The online software psRNATarget was used to predict target genes of plant miRNAs in virus genomes.The results showed that the target genes included coat protein,reverse transcriptase,republication related protein,readthrough protein and many other genes.

关 键 词：MIRNA 植物病毒 靶基因预测 生物信息学 

分 类 号：Q752[生物学—分子生物学]