去白细胞悬浮红细胞储存过程中细胞膜损伤变化  

Storage lesion of membrane of suspended erythrocyte without leukocyte during storage

作　　者：王素玲[1] 何路军[1] 韩卫[1] 何天博[2] 王艳彬[1] 王切[3] 

机构地区：[1]河北省血液中心检验科,石家庄050071 [2]河北医科大学第四医院检验科,石家庄050000 [3]河北医科大学解剖教研室,石家庄050017

出　　处：《国际输血及血液学杂志》2015年第6期486-491,共6页International Journal of Blood Transfusion and Hematology

基　　金：河北省科技厅科技支撑计划项目（13277717D）

摘　　要：目的 观察去白细胞悬浮红细胞随着储存时间延长,其细胞膜损伤变化情况.方法 选择2013年5～6月于河北省血液中心制备的去白细胞悬浮红细胞制剂中,采用简单随机抽样方法抽取6份去白细胞悬浮红细胞制剂标本为研究对象.于储存后第7,14,28及42天,采用扫描电子显微镜观察去白细胞悬浮红细胞的超微结构,并随机选取4个视野计算双凹圆盘状红细胞和棘状红细胞比例,采用全自动血细胞分析仪测定其平均红细胞体积（MCV）.采用化学比色法测定去白细胞悬浮红细胞外游离血红蛋白（FHb）含量,并采用统计学方法对其储存过程中去白细胞悬浮红细胞MCV及细胞外FHb含量随着储存时间变化情况进行相关性分析.结果 去白细胞悬浮红细胞随着储存时间延长的变化趋势如下：①随着储存时间延长,双凹圆盘状去白细胞悬浮红细胞数量逐渐减少,棘状红细胞的数量却逐渐增多.②去白细胞悬浮红细胞储存后第7,14,28及42天时,其MCV呈逐渐增大趋势.第42天时,其MCV显著大于第7,14及28天时,且差异均有统计学意义（P＜0.01,0.01,0.05）.去白细胞悬浮红细胞MCV与储存时间呈正相关关系（r=0.899,P＜0.01）.③去白细胞悬浮红细胞储存后第7,14,28及42天时,去白细胞悬浮红细胞外FHb含量呈逐渐升高趋势.第42天时,其含量显著高于第7,14及28天时,且差异均有统计学意义（P＜0.01）.去白细胞悬浮红细胞外FHb含量与储存时间呈正相关关系（r=0.969,P＜0.01）.结论 随储存时间延长,大部分去白细胞悬浮红细胞的正常形态逐渐消失,MCV增大,细胞外FHb含量升高.这表明,去白细胞悬浮红细胞的细胞膜随储存时间延长受损程度逐渐加重.Objective To investigate the risk of storage lesion of suspended erythrocyte membrane without leukocyte with prolonged storage time.Methods From May to June 2013,6 bags of suspended erythrocyte without leukocyte which were prepared at Hebei Blood Center were collected as research objects in this study by random sampling method.Onday 7,14,28 and 42 of storage,the ultrastructure changes of suspended erythrocyte without leukocyte were observed by scanning electron microscopy.Four views were selected randomly to count double concave disc suspended erythrocyte and suspended erythrocyte with spines.The mean corpuscular volume （MCV） of suspended erythrocyte without leukocyte was detected with blood cell analyzer,and the content of free hemoglobin （FHb） outside suspended erythrocyte without leukocyte was detected with chemical colorimetry.Correlation analysis between MCV,FHb concentration outside of suspended erythrocyte without leukocyte and storage time was carried out by statistical methods.Results The changes of suspended erythrocyte without leukocyte were as followed.①With the extension of storage time,the number of double concave disc suspended erythrocyte without leukocyte reduced gradually,but the number of suspended erythrocyte with spines increased gradually.②On day 7,14,28 and 42 of storage,MCV of suspended erythrocyte without leukocyte showed a significant increasing trend.And the MCV of suspended erythrocyte without leukocyte on day 42 was significantly higher than that on day 7,14 and 2 8 （P〈0.01,0.01,0.05）.There was a positive correlation between the MCV of suspended erythrocyte without leukocyte and its storage time （r=0.899,P〈0.01）.③On day 7,14,28 and 42 of storage,FHb concentration outside suspended erythrocyte without leukocyte showed a significant increasing trend,FHb concentration outside suspended erythrocyte without leukocyte on day 42 was significantly higher than that on day 7,14 and 28 （P〈0.01）.There was a positive correlation between the FHb concentration outside

关 键 词：红细胞 细胞膜 平均红细胞体积 血红蛋白 游离 超微结构 

分 类 号：R457.1[医药卫生—治疗学]