黄芪甲苷对异丙肾上腺素诱导的H9C2心肌细胞凋亡的影响  

Effects of astragaloside IV on isoproterenol induced H9C2 cardiomyocyte apoptosis

作　　者：梅蒙[1] 王洪新[1] 唐富天[1] 鲁美丽[1] 胡进[1] 许崇花 韩镕徽 

机构地区：[1]辽宁医学院辽宁省心脑血管药物研究重点实验室,锦州121001

出　　处：《中药药理与临床》2015年第5期37-41,共5页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家自然科学基金(81374008)

摘　　要：目的:探讨黄芪甲苷(Astragaloside IV,ASIV)对异丙肾上腺素(Isoproterenol,ISO)诱导的H9C2心肌细胞凋亡的抑制作用及机制。方法:H9C2心肌细胞传代后随机分为正常对照组、异丙肾上腺素(10μmol/L)组、普萘洛尔(2μmol/L)组和黄芪甲苷(25、50、100μmol/L)组,采用流式细胞术检测H9C2心肌细胞凋亡率;运用JC-1荧光探针检测各组细胞线粒体膜电位的变化;采用Western Blot分别测定线粒体和胞浆中细胞色素C(Cytochrome C,Cyt-C)的蛋白表达。结果:与正常对照组相比,异丙肾上腺素组的凋亡率显著增加,线粒体膜电位降低,细胞色素C外流,线粒体细胞色素C表达减少而胞浆中表达增多。与模型组相比,黄芪甲苷(50、100μmol/L)组能明显降低凋亡率,提高线粒体膜电位及减少细胞色素C外流,且呈一定剂量依赖性。结论:黄芪甲苷可通过线粒体凋亡途径抑制异丙肾上腺素诱导的心肌细胞凋亡。Aim： To explore the inhibitory effect of astragaloside IV on H9C2 cardiomyocyte apoptosis induced by isoproterenol and the possible mechanisms. Methods： H9C2 cells were randomly divided into control group,isoproterenol group（ 10μmol / L）,propranolol group（ 2μmol /L） and astragaloside IV（ 25,50,100μmol / L） groups. Flow cytometry was used to detect the apoptotic rate of H9C2 cells. JC-1 probes wereused to measure the mitochondrial membrane potential of H9C2 cells. Western Blot was used to quantify the cytochrome C protein expression in both mitochondrial and cytoplasm. Results： Compared to the control group,isoproterenol treatment significantly increased the apoptotic rate,decreased mitochondrial membrane potential,down-regulated mitochondrial cytochrome C expression and up-regulated cytoplasm cytochrome C expression. Compared to isoproterenol group,astragaloside IV（ 50,100μmol / L） combination markedly reduced apoptosis rate,remained mitochondrial membrane potential and inhibited cytochrome C efflux from mitochondrial. Moreover,the effect of astragaloside IV was dose dependent. Conclusion： Astragaloside IV exerts an inhibitory effect on isoproterenol mediated apoptosis,which is partly related to mitochondrial apoptosis pathway.

关 键 词：黄芪甲苷 异丙肾上腺素 线粒体 凋亡 

分 类 号：R285[医药卫生—中药学]