黄癸素对ECV304细胞的增殖、迁移和小管形成的影响  

The effects of HB on the proliferation,migration and tube-like structure formation of vascular endothelial cell

作　　者：祁蕙 林菁[2] 

机构地区：[1]福建省立金山医院,福州350028 [2]福建医科大学药学院,福州350108

出　　处：《中药药理与临床》2015年第5期41-44,共4页Pharmacology and Clinics of Chinese Materia Medica

摘　　要：目的:探讨黄癸素(HB)在体外对血管生成的影响。方法:MTT法测定黄癸素对ECV304增殖的影响,划痕法检测黄癸素对ECV304细胞迁移的影响,二维培养观察黄癸素对ECV304细胞小管样结构形成的影响。结果:MTT法测得黄癸素对ECV304细胞24、48、72小时的IC50分别为40.61、7.15和2.40mg/L;划痕法测得黄癸素浓度为1.25、2.5、5mg/L时的迁移抑制率分别为39%、53%和79%;与阴性对照组比较,经黄癸素处理的ECV304细胞小管数目均显著减少,且管腔不完整,浓度为20、30、40mg/L时小管形成抑制率分别为26.99%、39.26%和65.64%。结论:黄癸素可直接抑制ECV304细胞的增殖、迁移和小管样结构的形成,抑制血管新生,从而可能对肿瘤血管的形成起到间接抑制作用。Objective： To investigate the effect of HB（ berberine α-hydroxy-δ-decanoylethyl sulfonate） on neoangiogenesis. Methods： MTT assay was employed to determine the proliferation of vascular endothelial cells（ ECV304）. The wound scratch assay was applied to determine the migration capacity of ECV304 cells. Tube formation was evaluated by two-dimensional collagen gel assay. Results： HB inhibited the proliferation of ECV304 cells in a time- and concentration-dependent manner,with IC50 value of 40. 61,7. 15 and 2. 40 mg / L respectively when the cells were treated with HB for 24,48 and 72 h. The migration of ECV304 cells was reduced by 38. 56%,53. 11% and 78. 81%（ P〈0. 01）respectively when the cells incubated with 1. 25,2. 5 and 5 mg / L of HB. HB inhibited the tube-like structure formation at 20,30 and 40 mg / L for 18 h,with the inhibition ratio of 26. 99%,39. 26% and 65. 64%（ P〈0. 05,P〈0. 01 and P〈0. 01,respectively）. Conclusion：HB can effectively inhibit angiogenesis in vitro by suppressing the proliferation,migration and tube-like structure formation of vascular endothelial cell,and it may play an indirect inhibition to tumor angiogenesis.

关 键 词：黄癸素 ECV304细胞 细胞增殖 细胞迁移 小管形成 

分 类 号：R285[医药卫生—中药学]