脓肿分枝杆菌实验室快速检测方法的应用  

Rapid laboratory identification of Mycobacterium abscessus

作　　者：汪一萍 鲁勇 应建飞 王凤军 俞万钧 张艳[3] 

机构地区：[1]宁波市鄞州人民医院检验科,315040 [2]宁波市鄞州人民医院呼吸科 [3]浙江省杭州市红十字会医院结核病诊疗中心结核实验室

出　　处：《中华检验医学杂志》2015年第12期861-864,共4页Chinese Journal of Laboratory Medicine

摘　　要：目的评估比较应用HAIN基因分型试剂盒、基因芯片技术以及热休克蛋白65（hsp65）基因测序技术3种方法鉴定脓肿分枝杆菌的临床价值。方法回顾性收集2014年1月至2015年1月，宁波市鄞州人民医院微生物室临床分离13株非结核分枝杆菌，同时以HAIN结核鉴定药敏分子检测系统试剂盒、基因芯片技术及hsp65基因测序方法鉴定菌株，并以hsp65基因测序方法为对照，对3种方法鉴定进行比较评价。结果13例非结核分枝杆菌经HAIN基因分型试剂盒鉴定结果和hsp65基因测序方法鉴定结果一致，均为脓肿分枝杆菌；基因芯片技术鉴定结果为龟／脓肿分枝杆菌复合群（包括脓肿分枝杆菌和龟分枝杆菌），只能鉴定至复合群水平，不能具体区分至亚种类型。结论HAIN基因分型试剂盒与hsp65基因测序法测定结果一致并能够鉴定到种水平，但HAIN基因分型试剂盒操作更简便。Objective To identify Mycobacterium abscessus rapidly with HAIN molecular assay genotype kit, gene chip and hsp65 gene sequencing, and to assess the clinical value of these three methods. Methods 13 clinical non-tuberculous Mycobacterium （NTM） of in-patient samples were collected from January2014 to January 2015, in the Department of Clinical Laboratory, Yinzhou People＇s Hospital, and meanwhile, these strains were identified with HAIN molecular assay genotype Mycobacterium kit and gene chip respectively. The hsp65 gene sequencing was used as the standard method to be compared with HAIN and gene chip. Results The results of HAIN kit and hsp65 gene sequencing showed that all the 13 strains were subspecies Mycobacterium abscessus, while that of gene chip showed that these strains were Mycobacteriumchelonae complex strains, and the subtypescould not be identified. Conclusion These results obtained from the HAIN molecular assay genotype mycobacterium system are in agreement with those obtained from the hsp65 gene sequencing, whereas the HAIN kit method is easier to use.

关 键 词：非结核分枝杆菌 基因型 试剂盒 诊断 热休克蛋白质类 寡核苷酸序列分析 

分 类 号：R440[医药卫生—诊断学]