LC/MS测定血液中罗哌卡因、咪达唑仑与奥美拉唑  

Determination of ropivacaine,midazolam and omeprazole in blood by liquid chromatography-mass spectrometry

作　　者：张园[1] 王楠楠[1] 张美玲[1] 徐彩裙[1] 林丹[1] 王贤亲[1] 

机构地区：[1]温州医科大学司法鉴定中心法医毒物鉴定室,浙江温州325035

出　　处：《中国法医学杂志》2015年第6期587-589,共3页Chinese Journal of Forensic Medicine

摘　　要：目的建立一种灵敏、简单测定血液中罗哌卡因、咪达唑仑与奥美拉唑的液相色谱-质谱联用法。方法以卡马西平为内标,样品采用乙酸乙酯作溶剂,液-液萃取的方法。色谱柱为Zorbax SB-C18（2.1 mm×50 mm,5μm）,流动相为乙腈-0.1%甲酸,梯度洗脱,流速为0.4 m L/min,柱温30℃。采用ESI离子源,SIM离子方式监测。结果罗哌卡因、咪达唑仑均在5-2 000 ng/m L浓度范围线性良好（r〉0.996）,奥美拉唑在50-2 000 ng/m L浓度范围线性良好（r〉0.998）。罗哌卡因、咪达唑仑与奥美拉唑的方法回收率均在92%-112%,日内,日间RSD均小于15%。结论本方法可简单、高效地检测血液中罗哌卡因、咪达唑仑与奥美拉唑浓度。Objective To develop a sensitive and simple liquid chromatography- mass spectrometry method for determination of ropivacaine, midazolam and omeprazole in blood. Methods Carbamazepine was used as internal standard, ropivacaine, midazolam and omeprazole in blood were extracted from blood by liquid-liquid extraction with ethyl acetate, and then analyzed on a Zorbax SB-C18 （2.1 mm×50mm, 5 μm） column. The mobile phase consisted of aeetonitrile-0.1% formic acid with gradient elution at a flow rate of 0.4 mL/min, the column temperature was set at 30℃. LC-MS was performed in ESI source with SIM mode for quantification. Results The linear range of the concentration were 5 -2 000 ng/mL for ropivacaine and midazolam （ r 〉 0. 995 ）, 50 - 2 000 ng/mL for omeprazole （ r 〉 0. 998 ）. The relative recoveries of ropivaeaine, midazolam and omeprazole were in the range of 92% - 112%. The RSDs of intra-days and inter-day were both less than 15%. Conclusion The method is simple, quick, sensitive, it could be used for determination of ropivacaine, midazolam and omeprazole in blood samples.

关 键 词：法医毒物分析 液-质联用法 罗哌卡因 咪达唑仑 奥美拉唑 血液 

分 类 号：D919.1[医药卫生—法医学]