Overexpression of potato miR482e enhanced plant sensitivity to Verticillium dahliae infection  

作　　者：Liu Yang Xiaoying Mu Chao Liu Jinghui Cai Ke Shi Wenjiao Zhu Qing Yang 

机构地区：[1]College of Life Sciences, Nanjing Agricultural University [2]Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences

出　　处：《Journal of Integrative Plant Biology》2015年第12期1078-1088,共11页植物学报（英文版）

基　　金：supported financially by grants from the National Natural Science Foundation of China (11171155);the National Pear Industry Technology System (CARS-29);a Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions: Modern horticultural science (PAPD)

摘　　要：Verticillium wilt of potato is caused by the fungus pathogen Verticillium dahliae. Present sRNA sequencing data revealed that miR482 was in response to V. dahliae infection, but the function in potato is elusive. Here, we characterized potato miR482 family and its putative role resistance to Verticillium wilt. Members of the potato miR482 superfamily are variable in sequence, but all variants target a class of disease-resistance proteins with nucleotide binding site （NBS） and leucine-rich repeat （LRR） motifs. When potato plantlets were infected with V. dahliae, the expression level of miR482e was downregulated, and that of several NBS-LRR targets of miR482e were upregulated. Transgenic potato plantlets overexpressing miR482e showed hypersensitivity to V. dahliae infection. Using sRNA and degradome datasets, we validated that miR482e targets mRNAs of NBS-LRR disease-resistance proteins and triggers the production of trans-acting （ta）- siRNAs, most of which target mRNAs of defense-relatedproteins. Thus, the hypersensitivity of transgenic potato could be explained by enhanced miR482e and miR482e-derived ta- siRNA-mediated silencing on NBS-LRR-disease-resistance pro- teins. It is speculated that a miR482-mediated silencing cascade mechanism is involved in regulating potato resistance against V. dahliae infection and could be a counter defense action of plant in response to pathogen infection.Verticillium wilt of potato is caused by the fungus pathogen Verticillium dahliae. Present sRNA sequencing data revealed that miR482 was in response to V. dahliae infection, but the function in potato is elusive. Here, we characterized potato miR482 family and its putative role resistance to Verticillium wilt. Members of the potato miR482 superfamily are variable in sequence, but all variants target a class of disease-resistance proteins with nucleotide binding site （NBS） and leucine-rich repeat （LRR） motifs. When potato plantlets were infected with V. dahliae, the expression level of miR482e was downregulated, and that of several NBS-LRR targets of miR482e were upregulated. Transgenic potato plantlets overexpressing miR482e showed hypersensitivity to V. dahliae infection. Using sRNA and degradome datasets, we validated that miR482e targets mRNAs of NBS-LRR disease-resistance proteins and triggers the production of trans-acting （ta）- siRNAs, most of which target mRNAs of defense-relatedproteins. Thus, the hypersensitivity of transgenic potato could be explained by enhanced miR482e and miR482e-derived ta- siRNA-mediated silencing on NBS-LRR-disease-resistance pro- teins. It is speculated that a miR482-mediated silencing cascade mechanism is involved in regulating potato resistance against V. dahliae infection and could be a counter defense action of plant in response to pathogen infection.

关 键 词：miR482 nucleotide binding site leucine-rich repeat 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]