高压氧对创伤性颅脑损伤大鼠神经保护作用及骨髓间充质干细胞归巢因子表达的影响  

Effect of hyperbaric oxygen on the protection of nerve system and the expression of mesenchymal stem cells homing factor in rats with traumatic brain injury

作　　者：周苏键 谭耀武[1] 刘咏武栎 丁政[1] 卢晓欣[1] 彭慧平[1] 

机构地区：[1]南京军区福州总医院,福州350025

出　　处：《中华航海医学与高气压医学杂志》2015年第5期347-351,共5页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

基　　金：南京军区福州总医院专项基金（2011-009）

摘　　要：目的 观察高压氧（hyperbaric oxygen,HBO）对创伤性颅脑损伤（traumatic brain injury,TBI）大鼠受损脑组织中基质细胞衍生因子-1（stromal derived factor-1,SDF-1）及其趋化因子受体4（CXC chemokine receptor 4,CXCR4）表达的影响,探讨高压氧对创伤性颅脑损伤大鼠神经保护作用的潜在机制.方法 采用改良Feeney自由落体法复制建立颅脑损伤模型,SD雄性大鼠72只,按数字表法随机分为3组,假手术组、模型组、高压氧组,每组各24只,各组根据高压氧干预时间,再分为3d和10d2个亚组.高压氧组大鼠于造模后24 h接受高压氧处理,每日1次.在造模后24 h、处理3d、10 d后,运用神经功能评分（neurological severity scores,NSS）比较各组神经功能恢复情况.同时在3d、10 d时,取伤侧脑组织应用蛋白免疫印迹法（Western blotting）检测受损脑组织SDF-1及其受体CXCR4的表达情况.结果 （1）假手术组大鼠未见神经功能缺损,模型组与高压氧组大鼠造模24 h后神经功能缺损评分差异无统计学意义（P＞0.05）,治疗3d、10d,神经功能逐渐恢复,高压氧组神经功能缺损评分（分别为8.7±0.4、4.7±0.6）均明显低于模型组（分别为10.5±0.6、6.4±0.6）,差异有统计学意义（P＜0.01）.（2）假手术组SDF-1及CXCR4蛋白表达量少,不同时间点表达量差异无统计学意义（P＞0.05）;治疗3d,模型组与假手术组相比,SDF-1及CXCR4蛋白表达量均明显增多（P＜0.01）;治疗10d,模型组SDF-1表达下降,趋于假手术组水平（P＞0.05）,CXCR4表达下降,但仍明显高于假手术组（P＜0.01）;治疗3d、10 d,高压氧组和模型组比较,SDF-1及CXCR4表达量均明显增多（P＜0.01）.结论 HBO治疗可加快TBI大鼠神经功能恢复,可能通过上调SDF-1/CXCR4轴,从而促进内源性骨髓间充质干细胞归巢至受损脑组织.Objective To observe the effects of hyperbaric oxygen （HBO） on the expressions of stromal derived factor-1 （SDF-1） and CXC chemokine receptor 4（CXCR4） in rats with traumatic brain injury and also to discuss potential mechanism of HBO in the protection of the nerve system of the rats with TBI.Methods The experimental models of traumatic brain injury were developed by modified Feeney free-falling method.Seventy-two SD male rats were randomly divided into 3 groups： the sham surgery group, the model group and the HBO group, each consisting of 24 rats.Then, in accordance with HBO intervention time, the HBO group was subdivided into the 3-day and 10-day subgroups.The rats in the HBO group received HBO therapy 24 hours after development of the model, one session a day.Recovery of the nerve system, 24 hours after development of the model, 3 and 10 days after therapy, was assessed by using Neurological Severity Scores （NSS）, and neurological function recovery was compared between the groups.At the same time, samples of damaged brain tissues were taken for the detection of expression levels of SDF-1 and CXCR4 by using Western blotting.Results （1）Neurological function deficiency was not noted in the sham surgery group.There were no significant differences in neurological function deficiency scores detected 24 hours after the development of the model, when comparisons were made between the model group and the HBO group（P 〉0.05）.Three and 10 days after therapy, neural function recovered gradually, with the neural function deficiency scores of the HBO group being（8.7 ± 0.4）and（4.7 ±0.6）respectively, which were obviously lower than those of the model group（10.5 ±0.6 and 6.40.6 respectively）（P 〈0.01）.（2）The expressions of SDF-1 and CXCR4 in the sham surgery group were low, and there was no statistical significance in the expression levels at different time points（P 〉0.05）.Three days after therapy, the expression levels of SDF-1 and CXCR4 for the model group were all sign

关 键 词：高压氧 创伤性颅脑损伤 神经功能评分 细胞基质衍生因子-1 趋化因子受体4 归巢 大鼠 

分 类 号：R651.15[医药卫生—外科学]