中国东海冈村凹顶藻的化学成分及其生物活性研究  

Chemical constituents and bioactivities of Laurencia okamurai from the East China Sea

作　　者：杨欣欣[1,2] 谈吉 蔡厚才[1,2] 蔡春尔[1,2] 何培民[1,2] 贾睿[1,2] 

机构地区：[1]上海海洋大学水产与生命学院海洋科学研究院 [2]南麂列岛国家海洋自然保护区管理局,上海201306

出　　处：《中国海洋药物》2015年第6期28-34,共7页Chinese Journal of Marine Drugs

基　　金：国家海洋高技术研究发展计划项目(2014AA093506);国家科技支撑项目(2012BAC07B03);上海市科委项目(10391901900);教育部留学回国人员科研启动基金资助

摘　　要：目的对采自中国东海南麂岛海域的海洋红藻冈村凹顶藻(Laurencia okamurai)进行系统的化学成分研究,并对得到的单体化合物进行抗菌活性筛选。方法采用正相硅胶柱色谱、凝胶Sephadex LH-20以及半制备高效液相色谱HPLC等层析手段对冈村凹顶藻的甲醇提取物进行了系统的分离纯化,同时运用1 H NMR、13 C NMR、EIMS等光谱技术手段对得到的化合物进行结构鉴定,并利用无乳链球菌、鳗弧菌、嗜水气单胞菌、塔氏弧菌、海豚链球菌、鮰爱德华和HN016对所得单体化合物进行抗菌活性测试。结果从冈村凹顶藻中分离得到了11个化合物,包括7个倍半萜化合物、1个甾体化合物、1个不饱和脂肪酸、1个脂类化合物和1个醛类化合物,分别是aplysin(1),debromolaurinterol(2),aplysinol(3),debromoisolaurinterol(4),3β-hydroperoxyaplysin(5),laurinterol acetate(6),Laurokomurenene A(7),cholesterol(8),棕榈油酸(9),octadec-3(E)-en-2-one(10)和(2E)-2-tridecyl-2-heptadecenal(11)。其中化合物2对无乳链球菌和鳗弧菌HLPF表现出强的特异性抑制活性,其余化合物均无明显的抗菌活性。结论本研究首次发现化合物2对无乳链球菌和鳗弧菌显示出很好的特异性抑制活性。Objective To study the chemical constituents of the red algaLaurencia okamurai collected from the East China Sea near Nanji Island,and investigate the antibacterial activity of pure compounds.Methods The compounds were isolated and purified upon column chromatography by silica gel,Sephadex LH-20 and semi-preparative HPLC,while their structures were identified on the basis of spectroscopic data analysis including 1 H NMR、13 C NMR、EIMS and so on.Antibacterial activity of the compounds was evaluated in settlement inhibition assays with S.agalactiae,V.anguillarum,A.hydrophila,V.Tubiashii,S.iniae,Edwardsiella ictaluri,and HN016.Results Eleven known compounds were isolated from the MeOH extract of L.okamurai.The structures were identified as aplysin（1）,debromolaurinterol（2）,aplysinol（3）,debromoisolaurinterol（4）,3β-hydroperoxyaplysin（5）,laurinterol acetate（6）,Laurokomurenene A（7）,cholesterol（8）,Palmitoleic Acid（9）,octadec-3（E）-en-2-one（10）and（2E）-2-tridecyl-2-heptadecenal（11）.Compounds 2 showed significant specific antibacterial activity against S.agalactiae and V.Anguillarum.The rest of the compounds showed no obvious antibacterial activity.Conclusion This was the first report on the specific antibacterial activities against S.agalactiae and V.Anguillarumabout compound 2.

关 键 词：冈村凹顶藻 次级代谢产物 倍半萜 抗菌活性 

分 类 号：R931[医药卫生—生药学] R284.1[医药卫生—药学]