SLY在猪链球菌性脑膜炎致病机理中的作用初探  

Initial Exploration of the Role of SLY In the Pathogenesis of Streptococcus suis Meningitis

作　　者：郭洋[1] 伊鹏霏[2] 李鹏[1] 何剑斌[1] 张涛[3] 韦旭斌[2] 穆祥[3] 

机构地区：[1]沈阳农业大学畜牧兽医学院,沈阳110161 [2]吉林大学动物医学学院,长春130062 [3]北京农学院中兽医重点实验室,北京102206

出　　处：《沈阳农业大学学报》2015年第6期684-690,共7页Journal of Shenyang Agricultural University

基　　金：国家自然科学基金项目(30972209)

摘　　要：为探索猪链球菌溶血素(SLY)在猪链球菌性脑膜炎致病机理中发挥的作用,采用纯化的SLY作用于大鼠脑微血管内皮细胞(RBMEC),利用LDH法测定其对内皮细胞的毒性,并用ELISA检测法测定其对内皮细胞IL-6分泌水平的影响,TEER值检测法测定其对内皮细胞通透性的影响。结果表明:SLY可诱导RBMEC产生细胞毒性,且其毒性与SLY浓度及作用时间呈正相关;SLY可诱导RBMEC异常分泌IL-6,引发RBMEC的炎症反应,24h内,SLY能刺激RBMEC产生过量IL-6,除2h时IL-6的分泌水平与空白对照组无显著差异外,其他各时间点均显著高于空白对照组(p<0.01),不同浓度的SLY刺激RBMEC 12h后能造成IL-6的过量分泌,各浓度组的IL-6分泌水平均显著高于空白对照组(p<0.01);SLY可造成RBMEC细胞间通透性的增加,在一定的作用浓度范围内,其通透性增加水平与SLY浓度及作用时间呈正相关,除2μg·m L-1和4μg·m L-1组对TEER值的影响差异不显著之外,其他各SLY刺激组对TEER百分比的降低在8h后均呈浓度依赖。SLY可通过多种途径造成RBMEC的损伤,是导致血脑屏障通透性增加的重要因素,本研究初步证明了其在猪链球菌性脑膜炎致病机理中发挥了重要作用。This study was performed to initial exploration on the role of suilysin（SLY） in the pathogenesis of Streptococcus suis（S, suis） meningitis. Rat Brain Microvascular Endothelial Cells（RBMEC） was stimulated with purified SLY, then the cytotoxicity determination was developed by Lactate dehydrogenaseassay, IL-6 levels induced with suilysin was carried out with ELISA assay, and the permeability of the RBMEC was measured with TEER value assay. Cytotoxicity to RBMEC was induced with SLY, and the toxicity was positively correlated with the concentration of SLY and the duration of stimulation. An abnormal secretion ofIL-6 was induced with SLY, and inflammatory reaction was triggerd;Increased permeability of RBMEC induced with SLY was observed, and the level of increased permeability was positively correlated with the concentration of SLY and the duration of stimulation in a certain range of the concentration. RBMEC injurycould be induced with SLY in various ways, which is an important factor leading to the increased permeability of the Blood-Brain Barrier, initially proved SLY played an important role in the pathogenesis of S.suismeningitis.

关 键 词：猪链球菌溶血素 猪链球菌性脑膜炎 脑微血管内皮细胞 LDH IL-6 TEER 

分 类 号：S816.7[农业科学—饲料科学]