纳米硫化锌标记衰老蛋白Prelamin A的应用研究  

Application of ZnS Nanoparticles in Labeling Prelamin A Protein

作　　者：吴都督[1] 陈稚[1] 郑博丹 刘新光[2] 

机构地区：[1]广东医学院药学院,广东东莞523808 [2]广东医学院衰老所,广东东莞523808

出　　处：《广东化工》2015年第23期37-38,共2页Guangdong Chemical Industry

基　　金：国家自然科学基金项目(No.31201036);广东省2014年优秀青年培养项目(No.4CX14068G);广东省2015年建设中医药强省科研项目(No.20151257);广东医学院科技发展项目(No.M2012009);广东医学院博士启动项目(No.B2014007);广东医学院建博科技创新团队项目(No.STIF201102);广东省大学生创新项目(No.201510571042;No.201510571024);广东省2014年人才培养模式创新实验区项目

摘　　要：通过水热合成法合成了纳米硫化锌,并考察了纳米硫化锌对HEK-293的细胞毒性及对衰老蛋白prelamin A的标记。结果表明,合成的纳米硫化锌粒径约为10 nm,并在浓度小于800μM,孵育时间小于8 h,对HEK-293细胞毒性较小。同时通过抗原和抗体的特异性结合,纳米硫化锌可实现对衰老蛋白prelamin A的标记。Modern society faces an increasing aging problem, and China is becoming an aging society gradually. Prelamin A is an important aging protein. It is found that if the C-terminal ofprelamin A can not be cleaved or is cleaved abnormally, it will cause aging. Zinc sulfide （ZnS） nanoparticles have been widely used as novel fluorescent probes in biosensor and bioimaging due to their unique optical and electrical properties. Therefore, in this paper, ZnS nanoparticles were prepared by hydrothermal synthesis method, and were used to label prelamin A through strong combination by antibody and antigen and endocytosis of cell. Moreover, the toxicity of QDs to HEK-293 cells was investigated by MTT technology. We expect our work could be helpful tbr a better understanding of prelamin A in cellular senescence.

关 键 词：硫化锌 纳米颗粒 

分 类 号：TB383[一般工业技术—材料科学与工程]