百里酚蓝荷移分光光度法测定注射用磷霉素钠含量  

Determination of fosfomycin sodium for injection based on charge-transfer reaction spectrophotometric method

作　　者：李俊[1] 张社利[1] 赵桐辉[2] 王国庆[3] 李全民[2] 

机构地区：[1]焦作师范高等专科学校,河南焦作454000 [2]河南师范大学化学化工学院,河南新乡453007 [3]郑州轻工业学院材料与化学工程学院,河南郑州450002

出　　处：《化学研究与应用》2015年第12期1885-1887,共3页Chemical Research and Application

基　　金：国家自然科学基金项目(21075113)资助;河南省科技厅软科学项目(122400450221)资助;焦作市科技局项目(SGG2013G0048;SJC20130004)资助

摘　　要：利用注射用磷霉素钠与百里酚蓝在乙醇介质发生荷移反应,建立了荷移分光光度法测定注射用磷霉素钠含量的方法。结果表明,荷移反应生成3∶1型配合物,最大吸收波长为441 nm,表观摩尔吸光系数为ε=9.21×103L·mol^(-1)·cm^(-1),注射用磷霉素钠测定线性范围为0.123~22.53 mg·L^(-1),线性回归方程为A=0.2070+0.03158C(C为mg·L^(-1)),用拟定的方法对注射用磷霉素钠进行了含量测定,结果与文献方法一致,平均回收率99.4%。A speetrophotometric method was established for the determination of fosfomyein sodium for injection base on its charge- transfer reaction with thymol blue in ethanol solution. The experimental results showed that the complex composition ratio is 3：1, the apparent molar absorption coefficient is 9. 21×10^3 L-mol^-1·cm^-l at maximum absorption wavelength 441 nm, and the linear range of fosfomyein sodium is 0. 123 - 22. 53mg·L^-l and the linear equation is A =0. 2070＋0. 03158C（ C,mg. L^-1）. The method was applied to the determination of fosfomycin sodium for injection and the results were consistent with the literature reported with average recovery of 99.4%.

关 键 词：注射用磷霉素钠 百里酚蓝 荷移反应 分光光度法 

分 类 号：O675.32[理学—化学]