乳化氟碳联合腺苷A_2a受体激动剂对离体肺的保护作用  

Protective Effect of Perfluorocarbon Emulsions Combine with Adenosine A_2a Receptor Agonist on Rat Donor Lung

作　　者：卢春英[1] 易艳萍 蒋德英[1] 席露[3] 刘嘉奕 彭雪梅[1] 

机构地区：[1]暨南大学第一附属医院麻醉科,广东广州510630 [2]广东省广州市人口和计划生育技术服务指导所,广东广州510410 [3]广州医科大学第三附属医院麻醉科,广东广州510140 [4]中山大学附属第二医院麻醉科,广东广州510120

出　　处：《中山大学学报（医学科学版）》2015年第6期852-856,共5页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：广东省科技计划项目(2060303)

摘　　要：【目的】观察乳化氟碳保存液联合A_2a受体激动剂对离体肺的保护作用。【方法】选择48只质量约250~280 g的雄性SD大鼠随机分为乳化氟碳组（FCE）、低钾右旋糖酐组（LPD）、乳化氟碳＋A_2a组（FCEA）和低钾右旋糖酐＋A_2a组（LPDA）4组,每组12只。麻醉后建立离体肺模型并取心肺组织置于相应保存液中,其中FCE组与LPD组的肺灌注保存液分别为FCE液和LPD液;FCEA组与LPDA组的肺灌注保存液为FCE与LPD分别和A_2a受体激动剂CGS21680（0.1 mg/kg）的混合液。6 h后取肺组织检测NOS、iNOS、MPO、SOD的活性和MDA含量的变化以及病理改变。【结果】LPD组与FCE组的NOS、iNOS、MPO、SOD、MDA值无统计学差异（P〉0.05）,LPDA组与FCEA组的MPO值无统计学差异（P〉0.05）,LPDA和FCEA组的NOS、iNOS、MPO值均低于FCE和LPD组,SOD值均高于FCE和LPD组（P〈0.05）,FCEA组的NOS、iNOS均低于LPDA组,SOD值均高于LPDA组（P〈0.05）,FCEA组的MDA含量及病理积分均低于其他3组（P〈0.05）。【结论】乳化氟碳保存液联合A_2a受体激动剂能提高肺的抗炎能力,减轻肺损伤。【Objectives】 To observe the effect of perfluorocarbon emulsion combined with A_2a agonists on rat donor lung.【Methods】 The 48 SD rats（male, weighing, 250 to 280 g） were randomly divided into four groups using a random number table ：perfluorocarbon emulsion group（FCE）, low potassium dextran（LPD）, fluorocarbon emulsion and A_2a agonists（FCEA） and low potassium dextran and A_2a agonists（LPDA）. We constructed the isolated lung models on all rats, then took out the whole of heart and lungs, stored in lung preservation solution. FCE groups were flushed and stored with FCE, LPD groups were flushed and stored with LPD solution, FCEA groups were flushed and stored with FCE and CGS21680（0.1 mg / kg） of the mixture, LPDA groups were flushed and stored with LPD and CGS21680（0.1 mg / kg） of the mixture. After 6 hours, Specimens were harvested and analyzing the activity of NOS, iNOS, MPO and SOD, the content of MDA and the histopathological changes. 【Results】 The NOS, iNOS, MPO,SOD and MDA were no significant difference between FCE and LPD groups（P〉0.05）. The activity of MPO were no difference between FCEA and LPDA groups（P〉0.05）. The activity of NOS, iNOS and MPO of the FCEA and LPDA groups were significantly lower than FCE and LPD groups, but SOD were significantly higher than（P〈0.05）. The activity of NOS and iNOS of FCEA group were significantly lower than LPDA group, but SOD were significantly higher than（P〈0.05）. The content of MDA and pathologicalscore of FCEA group were significantly lower than the other three groups（P〈0.05）. 【Conclusion】 Perfluorocarbon emulsion preservation solution combined with A_2a agonists may improve the pulmonary anti-inflammatory ability and abate lung injury.

关 键 词：乳化氟碳 A2a受体激动剂 低钾右旋糖酐 肺保护 

分 类 号：R617[医药卫生—外科学]