PCR扩增测序法寻找中国麻风杆菌新种Mycobacterium lepromatosis研究  

PCR amplification and sequencing in screening Mycobacterium lepromatosis in China

作　　者：温艳[1] 袁有华[2] 翁小满[1] 邢燕[1] 张颖[3] 

机构地区：[1]首都医科大学附属北京友谊医院麻风室北京热带医学研究所热带病防治研究北京市重点实验室,北京100050 [2]复旦大学附属华山医院感染科,上海631000 [3]约翰霍普金斯大学彭博公共卫生学院分子微生物和免疫系,美国巴尔的摩21205

出　　处：《中华实用诊断与治疗杂志》2015年第12期1163-1165,共3页Journal of Chinese Practical Diagnosis and Therapy

基　　金：热带病防治研究北京市重点实验室开放课题(2014RDBF04)

摘　　要：目的了解中国目前传播的麻风杆菌中是否存在麻风杆菌新种。方法对来自中国23个省市125例麻风患者皮损组织DNA,采用巢式PCR扩增麻风杆菌特异16SrRNA保守片段,PCR阳性产物直接测序,经BLAST进行序列比对。结果完成测序的86例菌株经BLAST比对发现,扩增片段与来自巴西的麻风杆菌Br4923相似性达99%,未发现麻风杆菌新种Mycobacterium lepromatosis;86例中64例16SrRNA序列的251位碱基存在变异(C-T),存在突变的菌株多为SNPⅢ型,不存在突变的为SNPⅠ和Ⅱ型。结论未发现麻风杆菌新种,16SrRNA基因序列突变C251T与SNP分型有关,可提示不同基因型麻风杆菌的地理分布。Objective To screen Mycobacterium lepromatosis in China by PCR amplification. Methods Totally 125 DNA samples from skin biopsies of leprosy patients from 23 provinces in China were amplified the 16S rRNA gene of Mycobacterium leprae by using nested-PCR, followed by DNA sequencing and BLAST. Results In 125 specimens, 86 were sequenced and compared with BLAST, showing 99% similarity with Brazil Br4923, and Mycobacteriurn leprornatosis was not found. A mutation at C251T in the 16S rRNA gene was found in 64 specimens. The 16S rRNA C251T mutation belonged to SNP type III , whereas strains without the point mutation belonged to SNP type I and II. Conclusion The new species Mycobacterium lepromatosis is not found. The 16S rRNA gene sequence mutation C251T is correlated with SNP typing, indicating a difference in the geographical distribution of the strains.

关 键 词：麻风杆菌 巢式PCR 16SrRNA MYCOBACTERIUM lepromatosis 

分 类 号：R755[医药卫生—皮肤病学与性病学]