辐射白刺花遗传多样性的ISSR和RAPD比较分析  

Comparison of Genetic Diversity of Irradiated Sophora vicii folia Using ISSR and RAPD Markers

作　　者：李凤涛[1] 赵丽丽[1] 王普昶 陈超[1] 吴小丽[1] 

机构地区：[1]贵州大学动物科学学院草业科学系,贵州贵阳550025 [2]贵州省草业研究所,贵州贵阳550025

出　　处：《草地学报》2015年第5期1114-1118,共5页Acta Agrestia Sinica

基　　金：贵州省科教青年英才培育工程项目[黔省专合字(2012)142号];国家自然科学基金(31260572);贵州大学引进人才科研项目[贵大人基合字(2010)036号];贵州大学教改项目(JG2013067)资助

摘　　要：采用ISSR和RAPD标记对8个^(60)Co-γ辐射梯度诱变白刺花(Sophora viciifolia)进行遗传多样性分析。结果表明:20条ISSR和6条RAPD引物的PPL多态率分别为51.37%和21.03%,ISSR的Nei's指数和平均Shannon指数均大于RAPD;ISSR的标记指数(1.28)是RAPD(0.20)的6.4倍,表明ISSR具有较高的标记效率。Mantel检测表明,ISSR+RAPD标记与ISSR标记的结果显著相关(P<0.05)。综合分析表明白刺花用1SSR标记可检测出更高的多态性,更能准确反映白刺花材料间的遗传关系。The RAPA and ISSR markers were used in analyzing the genetic diversity of mutagenic Sophora vicii folia Franch with eight different ∽（60）Co-γ-rays gradients.The results showed that the percentage of polymorphic bands of 20 ISSR primers and 6 RAPD primers were 51.27%and 21.03%,respectively.The Nei＇s gene heterozygosity and Shannon＇s information index of ISSR were greater than RAPD.For 8 materials,ISSRs got a MI value（1.28） that was 6.4 times higher than that of RAPDs（0.20）,indicating that ISSRs had a higher MI efficiency.The Mantel test revealed that the correlation was significant between ISSR＋RAPDs and ISSRs（r=0.9791,P0.05）.Comprehensive research showed that ISSRs could detect the higher polymorphism of Sophora vicii folia,and more accurately reflect the genetic relationship among Sophora vicii folia.

关 键 词：白刺花 辐射诱变 ISSR RAPD 遗传多样性 

分 类 号：S548[农业科学—作物学]