萜类化合物生物合成途径中关键酶的研究进展  

Research Advances of Key Enzymes in the Biosynthesis Pathways of Isoprenoids

作　　者：马转转[1] 庞潇卿 谌容[2] 裴晓林[3] 王秋岩[3] 谢恬[2] 殷晓浦[3] 

机构地区：[1]杭州师范大学生命与环境科学学院,浙江杭州310036 [2]杭州师范大学医学院,浙江杭州310036 [3]杭州师范大学材料与化学化工学院,浙江杭州310036

出　　处：《杭州师范大学学报（自然科学版）》2015年第6期608-615,共8页Journal of Hangzhou Normal University(Natural Science Edition)

基　　金：国家自然科学基金项目(21206024);浙江省自然科学基金项目(Y15B060012;Q15C020008;Y15B060045);杭州市重大科技专项(20142013A63);杭州市科技发展计划项目(20130432B05)

摘　　要：萜类化合物作为一种广泛存在的天然产物,在植物生命活动中发挥着重要作用,在医药和工业等领域也有着重要应用.它有甲羟戊酸途径和脱氧木酮糖-5-磷酸途径两条生物合成途径,羟甲基戊二酰CoA还原酶、1-脱氧木糖-5-磷酸合酶、1-脱氧木糖-5-磷酸还原异构酶、异戊烯基转移酶及萜烯合酶是这两条途径中调控萜类合成的关键酶.文章综述了这些关键酶基因克隆、表达、分类、作用机制及代谢调控等方面的研究进展.Isoprenoids are a large and diverse group of natural products which play important roles in plant metabolic activities.They are also widely used in medicine and industry field,which are synthesized via two distinct pathways,the mevalonate pathway and the 1-deoxy-D-xylulose-5-phosphate pathway. 3-hydroxy-3-methylglutaryl CoA reductase（HMGR）,1-deoxy-D-xylulose-5-phosphate synthase（DXS）,1-deoxy-D-lxylulose-5-phosphate reductoiso-merase（DXR）,isopentenyl transferases and terpene synthases are the key enzymes that regulate isoprenoids synthesis in the two pathways.The research advances of molecular cloning,genetic expression,classification,metabolism and metabolic engineering of these key enzymes were reviewed.

关 键 词：萜类化合物 生物合成 关键酶 

分 类 号：Q55[生物学—生物化学]