输血前ELISA法检测HBsAg、抗-HCV、抗-HIV及抗-TP的价值分析  

The Value of Detection and Analysis of HBs Ag,Anti-HCV,Anti-HIV and Anti-TP by ELISA before Blood Transfusion

作　　者：肖婕[1] 彭心华[1] 余艳婷[1] 

机构地区：[1]江西省吉安市妇幼保健院,江西吉安343000

出　　处：《中国医学创新》2015年第31期114-117,共4页Medical Innovation of China

摘　　要：目的:探讨输血前酶联免疫吸附试验(ELISA)法检测乙肝表面抗原(HBs Ag)、丙型肝炎病毒抗体(抗-HCV)、艾滋病病毒抗体(抗-HIV)、梅毒螺旋体抗体(抗-TP)的临床价值。方法:回顾性分析2014年2月-2015年2月在本院拟输血的1026例患者的临床资料,均在输血前进行酶联免疫吸附试验(ELISA)法检测,研究乙肝表面抗原(HBs Ag)、HBs Ag、丙型肝炎病毒抗体(抗-HCV)、艾滋病病毒抗体(抗-HIV)、梅毒螺旋体抗体(抗-TP)检测阳性率,并进行分析。结果:1026例患者中输血前Hbs Ag检测验性率最高,达9.94%,抗-HCV、抗-HIV、抗-TP分别为1.75%、0.19%、2.24%,不同指标阳性率进行比较差异有统计学意义(字2=278.14,P<0.05)。对不同指标阳性率的科室分布情况进行统计和比较,病理产科的阳性率最高,达29.66%,不同科室的相关指标阳性率分布情况进行比较差异有统计学(字2=73.52,P<0.05)。结论:输血前各种疾病存在一定的感染率,其中乙肝感染率较高,且在不同科室的分布情况存在一定的差异,利用ELISA法进行输血前HBs Ag、抗-HCV、抗-HIV及抗-TP检测十分有必要。Objective：To explore the clinical value of enzyme linked immunosorbent assay （ELISA） method for detection of hepatitis B surface antigen （HBsAg）,hepatitis C virus antibody （anti-HCV）,AIDS virus antibody （anti-HIV） and treponema pallidum antibody （anti–TP） before the blood transfusion.Method：A retrospective analysis was performed on clinical data of 1026 patients with blood transfusion institute in our hospital from February 2014 to February 2015 and they were tested by the enzyme linked immunosorbent assay （ELISA） method before blood transfusion.The positive detection rate of hepatitis B surface antigen （HBsAg）,HBsAg,hepatitis C virus antibody （anti-HCV）,AIDS virus antibody （anti -HIV） and treponema pallidum antibody （anti-TP） were researched and analyzed.Result：HbsAg detection test was the highest of 9.94% in 1026 patients before blood transfusion,anti-HCV,anti-HIV and anti-TP was respectively 1.75%,0.19% and 2.24%, compared with the positive rate of different indicators, the difference was statistically significant（ χ2=278.14,P〈0.05）.Statistical analysis and comparison to the distribution department of the positive rate of different indicators, the positive rate in the pathological obstetrics was the highest of 29.66%, compared different departments’ relevant indicators of positive rate distribution, the difference was statistically significant （ χ2=73.52,P〈0.05）.Conclusion：There are some diseases before blood transfusion infection rate, the infection rate of hepatitis B is high,and there are some differences in the distribution of different departments,it is necessary to use the method of ELISA before blood transfusion HBsAg,anti-HCV, anti-HIV and anti-TP detection.

关 键 词：输血前检测 酶联免疫吸附试验 乙肝表面抗原 丙型肝炎病毒抗体 艾滋病病毒抗体 梅毒螺旋体抗体 

分 类 号：R446.6[医药卫生—诊断学]