BACTEC-MGIT 960快速药敏与罗氏比例法药敏检测方法对比  

Drug sensitivity comparison between BACTEC- MGIT 960 and Roche proportion

作　　者：杨顺利[1] 布红丽 范梦柏[1] 

机构地区：[1]山西省结核防治中心太原市第四人民医院,山西太原030053

出　　处：《临床医药实践》2015年第12期919-921,共3页Proceeding of Clinical Medicine

摘　　要：目的：比较BACTEC-MGIT960全自动分枝杆菌快速药敏与罗氏比例法药敏检测方法的一致性。方法：平行检测住院患者分枝杆菌临床分离株245株的利福平（RFP）、异烟肼（INH）、链霉素（SM）、乙胺丁醇（EMB）的药物敏感性。结果：经对比检测,BACT-MGIT960全自动分枝杆菌药敏测定可在4-13 d内检测RFP,INH,SM,EMB的药物敏感性,平均7.8d。与罗氏比例法药敏的符合率分别达到98.78%,97.55%,96.73%,96.73%。平均符合率为97.45%。结论：BACTEC-MGIT 960分枝杆菌液体药敏系统,可快速准确获得药敏检测结果,尤其适用于临床分离株的快速药敏筛检。Objective： To compare the consistency of the method for the detection of the fast drug sensitivity of BACTEC-MGIT960 and the ratio of Roche to the drug sensitivity test. Methods： Parallel detection of the hospitalized patients with Mycobacterium tuberculosis drug sensitivity of separated strains 245 strains of rifampicin（ RFP）,isoniazid（ INH）,streptomycin（ SM） and ethambutol（ EMB）. Results： By contrast detection,BACT- MGIT960 automatic mycobacteria drug sensitivity determination can inspect the drug sensitivity of RFP,INH,SM,EMB in the 4 - 13 d and average 7. 8 days. The coincidence rate of drug sensitivity was 98. 78%,97. 55%,96. 73%,96. 73%,respectively. The average coincidence rate was 97. 45%,and the correlation was 97. 5%. Conclusion： BACTEC- MGIT 960 liquid drug sensitive system,can quickly and accurately obtain drug sensitivity test results,especially for clinical isolates of rapid drug sensitive screening.

关 键 词：MGIT960系统 药敏检测 比例法 结核分枝杆菌 

分 类 号：R446.5[医药卫生—诊断学]