Tween60和SDS强化白腐真菌修复DDT污染土壤  

Enhancement of Tween 60 and SDS for bioremediation of DDT-contaminated soil by white rot fungus

作　　者：肖鹏飞[1] 李玉文[1] KONDO Ryuichiro 

机构地区：[1]东北林业大学林学院,黑龙江哈尔滨150040 [2]Faculty of Agriculture,Kyushu University

出　　处：《中国环境科学》2015年第12期3737-3743,共7页China Environmental Science

基　　金：中央高校基本科研业务费专项资金项目(DL13CB12);国家自然科学基金项目(41201307);日本农林水产省农产品安全项目(PO-3216);人社部留学回国人员科技活动择优资助项目

摘　　要：为了提高DDT污染土壤的修复效果,研究了非离子表面活性剂Tween60及阴离子表面活性剂SDS在单一和组合两种方式对人工污染黑土中白腐真菌Phlebia lindtneri GB1027降解去除DDT的强化作用.结果表明,Tween60和SDS均能不同程度地促进土壤中DDT的生物降解,尤其在两种表面活性剂浓度为1.Omg/g干土时,土壤中DDT的降解率分别达到最高的62.9%和53.9%.相同浓度下,Tween60比SDS更有利于提高DDT污染土壤的生物修复效果,去除率提高接近10%将Tween60与SDS以不同质量比例组合处理后的DDT去除率大小为:Tween60-SD S(3:1)>Tween60-SD S(2:1)>Tween60-SD S(1:1),尤其在质量比为3:1和2:1时的DDT去除率甚至高于Tween60单独处理时的去除率,表明将两种类型的表面活性剂组合后对提高DDT的生物可利用性产生了协同效果研究还发现,菌株接种量越高,土壤中DDT的降解去除率越高,当接菌量达到1.OmL/g干土时,DDT的30d去除率达到最高的70.9%.在土壤含水率为10%-50%范围内,白腐真菌对DDT的降解去除效果随着土壤含水率的升高而增加,当土壤含水率达到50%时,土壤中有约70%的DDT被降解去除.本研究结果进一步证实了利用表面活性剂强化白腐真菌修复有机污染土壤的可行性.The effects of nonionic surfactant Tween60 and anionic surfactant SDS on bioremediation of DDT contaminated soils by white rot fungus Phlebia lindtneri GB1027 were investigated. The results showed that Tween 60 and SDS could enhance the degradation rate of DDT by fungus in soil, and the optimum concentration of both surfactants for fungal degradation of DDT was 1.0mg/g. P lindtneri GB1027 showed the higher degradation activity of DDT from soil with Tween60than that with SDS. The efficiency of Tween60-SDS mixed surfactants for enhancing biodegradation capacity was as： Tween60-SDS （3：1）〉Tween60-SDS （2：1）〉Tween60-SDS （1：1）, and the enhancing capacity of Tween60-SDS （3：1） and Tween60-SDS （2：1） for fungal degradation of DDT from soil was higher than that of Tween 60. The inoculum amount could greatly enhance the removal rate of DDT from soil by P lindtneri GB1027at a range of 0.2 to 1.0mL/g, and the highest degradation rate of DDT was 70.9% within 30days, when the inoculum amount of fungus was 1.0mL/g. It was also found that fungal degradation rates of DDT increased sharply with increasing soil moisture at a range between 10% and 50%, and that DDT of about 70% was removed from soil with moisture of 50% during 30 days of incubation. The above results confirmed the workability of white rot fungus-surfactants augmented remediation of DDT-contaminated soil.

关 键 词：DDT 表面活性剂 白腐真菌 生物降解 土壤修复 

分 类 号：X53[环境科学与工程—环境工程]