一种两亲性纤维素衍生物应用于毛细管电泳分离的研究  

A Novel Amphiphilic Cellulose Derivative for Capillary Electrophoresis Separation

作　　者：周海龙 黄迪惠 周平[1] 

机构地区：[1]生物医学分析化学教育部重点实验室武汉大学化学与分子科学学院,湖北武汉430072

出　　处：《分析科学学报》2015年第6期746-750,共5页Journal of Analytical Science

基　　金：湖北省自然科学基金(No.2014CFB179)

摘　　要：本文以纤维素为原料，合成了一种两亲性纤维素衍生物十二烷基疏水改性季铵化纤维素。将该衍生物用作毛细管电泳背景电解质的添加物，在pH=3．0～12．0的范围内能产生稳定的阳极电渗流，可应用于芳香酸和磺胺类药物的快速分离，对中性化合物烷基苯酮同系物也有一定的分离效果。实验结果表明，该两亲性纤维素衍生物不但能调节电渗流的大小和方向，而且可与分离对象发生疏水相互作用，可用于不同荷电性质化合物的电泳分离。Cationic cellulose derivative,quaternized cellulose（QC）,was homogeneously synthesized at first by modifying cellulose with etherifying agent.Dodecyl modified quaternized cellulose（DMQC）was then obtained by the further modification of QC.When DMQC was added in the background electrolyte as the additive,the capillaries generated stable reversed EOF over the pH range of 3.0-12.0.The salicylic acid,p-nitrobenzoic acid,p-aminobenzosulfonic acid and acetylsalicylic acid could be separated within 8min.Six sulfa drugs were separated in less than 10 min.In addition,neutral compounds,alkyl phenyl ketone homologues,could be partly separated.The results demonstrated that both the direction and the magnitude of the EOF were changed with the addition of DMQC,and the use of DMQC incorporated a possible hydrophobic interaction that improved the separation of neutral analytes.

关 键 词：纤维素衍生物 两亲性 毛细管电泳 

分 类 号：O657.8[理学—分析化学]