酸性刺激响应的二氧化硅纳米容器及其体外释放  

Acid Stimuli-responsive Silica Nano-container and Release in Vitro

作　　者：孙广平[1] 傅佳骏[1] 

机构地区：[1]南京理工大学化工学院,江苏南京210094

出　　处：《精细化工》2015年第12期1343-1349,共7页Fine Chemicals

基　　金：中央高校基本科研业务费专项资金资助(30915012207;30915011312)~~

摘　　要：研究了以缩酮链作为刺激响应单元的智能纳米容器在仿溶酶体和内涵体条件下的释放效果。以介孔二氧化硅纳米微球(MSNs)为载体,吸附荧光指示剂-罗丹明B,用2-(2-溴-乙氧基)-2-(2-叠氮基-乙氧基)丙烷作为连接链,将β-环糊精连接到介孔出口处进行封装,制备了酸性-刺激响应智能纳米容器,并用固体核磁共振(SSNMR)、傅里叶红外光谱(FTIR)和热重分析(TGA)验证了MSNs表面改性过程。使用荧光分光光度计实时监测智能纳米容器在不同pH下释放动力学曲线、紫外分光光度计测得该智能纳米容器的载药量为15.23 mg/g。结果表明,利用末尾封端技术制得的以缩酮链作为刺激响应单元的智能纳米容器实现了在中性条件下"零释放",酸性条件下大量释放(即仿溶酶体和内涵体条件下适量缓慢释放)的效果,此项研究在肿瘤细胞靶向方面具有很好的应用前景。In order to study the effect of intelligent nano-container＇s release function in imitation of the condition of Lysosome and Endosome, an acid stimuli-responsive intelligent nano-container using ketal chain as a stimuli-responsive unit was prepared using Mesoporous Silica Nanoparticles（MSNs） as a carrier, adsorbing fluorescent macromolecular Rhodamine B and encapsulated at the place of MSNs＇ s mesoporous by/3-cyclodextrin with 2- （ 2-bromo-ethoxy ） -2- （ 2-azido-ethoxy ） propane. The functionalized surface of MSNs was demonstrated by Nuclear Magnetic Resonance （NMR）, Fourier Transform Infrared Spectrometer （FTIR） and Thermo Gravimetric Analysis （TGA）. The releasing performances of Rhodamine B at different pH values were monitored by RF-5201 spectrophotometer and the drug loaded in the intelligent nano-container was 15.23 mg/g which was measured by Ultraviolet-Visible Spectrophotometer （UV-Vis）. The intelligent nano-containers constructed by assembly-technology realized ＂zero release＂ under neutral condition and remarkable release under acidic condition-- suitable release in imitation of the condition of Lysosome and Endosome and this research has extensive application prospects in terms of targeting tumor ceils.

关 键 词：介孔二氧化硅纳米微球 罗丹明B 酸性-刺激响应 功能材料 

分 类 号：O647[理学—物理化学] O645[理学—化学]