侧脑室注射M35对2型糖尿病大鼠心肌组织GLUT4表达的影响  

Effect of intracerebroventricular injection of M35 on GLUT4 expression in myocardial tissues of type 2 diabetic rats

作　　者：张真稳 方彭华[2,3] 史明仪 王艳 曹灵 卜平 朱妍 

机构地区：[1]扬州大学临床医学院,江苏扬州225001 [2]扬州大学江苏省中西医结合老年病防治重点实验室 [3]南京中医药大学翰林学院

出　　处：《山东医药》2015年第46期8-10,共3页Shandong Medical Journal

基　　金：国家卫生和计划生育委员会科研基金资助项目(W201309)

摘　　要：目的观察侧脑室注射中枢性甘丙肽(GAL)受体拮抗剂M35对2型糖尿病大鼠心肌组织葡萄糖转运蛋白4(GLUT4)表达的影响。方法取22只健康雄性Wistar大鼠制作2型糖尿病模型,将16只造模成功大鼠分为观察组和模型组各8只,另取8只健康大鼠为对照组。观察组于侧脑室注射M35 2μL,模型组和对照组分别于侧脑室注射生理盐水2μL,1次/d,均干预21 d。采用血糖钳实验测定各组葡萄糖输注速率,采用real-time PCR法检测心肌组织的GLUT4 mRNA。结果观察组、对照组、模型组的葡萄糖输注速率分别为(13.4±1.32)、(22.98±2.32)、(16.91±1.56)mg/min,P均<0.05;心肌组织GLUT4 mRNA的相对表达量分别为3.72±1.79、5.98±0.86、11.89±2.12,P均<0.05。结论侧脑室注射M35可使2型糖尿病大鼠心肌组织GLUT4的表达上调,从而提高心肌胰岛素敏感性。Objective To observe the influence of intracerebroventricular injection of galanin （ GAL） receptor antago-nist M35 on the expression of glucose transporter type 4 （GLUT4） in myocardial tissues of type 2 diabetic rats.Methods We took 22 healthy male Wistar rats to make the type 2 diabetic models .Sixteen model rats were successfully made and then were divided into the observation group and the model group , 8 rats in each group.Besides, another 8 healthy rats were selected as the control group .The intracerebroventricular injection of 2μL/d M35 was administered in the observation group, and 2 μL/d saline was injected in the model and control groups , once a day and both groups were treated for 21 days.The euglycemic-hyperinsulinemic clamp test was used to detect the glucose infusion rates .GLUT4 mRNA was detec-ted by real-time PCR.Results The glucose infusion rates in the observation group , control group and model group were re-spectively （13.4 ±1.32） mg/min, （22.98 ±2.32） mg/min and （16.91 ±1.56） mg/min, （all P〈0.05）.GLUT4 mR-NA expression levels in the myocardial tissues of the observation group , control group and model group were 3.72 ±1.79, 5.98 ±0.86 and 11.89 ±2.12, respectively （all P〈0.05）.Conclusion GAL can enhance the insulin sensitivity of my-ocardial tissues through the increased GLUT 4 expression of type 2 diabetic rats .

关 键 词：葡萄糖转运蛋白4 甘丙肽受体 2型糖尿病 心肌组织 胰岛素敏感性 

分 类 号：R587.1[医药卫生—内分泌]