苦瓜提取物对高脂肪喂食诱导的肥胖和胰岛抵抗C57BL/6j小鼠保护作用研究（英文）  

Momordica charantia Extract Protects C57BL /6j Mice from High-Fat-Diet-Induced Obesity and Insulin Resistance

作　　者：王日康[1] 赵婷婷 孙勇兵[1] 陈河如[3,4] 

机构地区：[1]江西中医药大学中药固体制剂制造技术国家工程研究中心,江西南昌330006 [2]河南省濮阳市妇幼保健院药学部,河南濮阳457000 [3]暨南大学药学院中药及天然药物研究所,广东广州510632 [4]广东省中药药效物质基础及创新药物研究重点实验室,广东广州510632

出　　处：《中山大学学报（自然科学版）》2015年第6期6-13,共8页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：国家自然科学基金资助项目(81172982);江西省自然科学基金资助项目(20151BAB215030)

摘　　要：通过高脂肪喂食诱导的C57BL/6j小鼠动物模型研究苦瓜提取物对肥胖和胰岛抵抗的保护作用。小鼠喂养2周后成功建立肥胖小鼠模型,随机分为正常对照组,肥胖小鼠模型组和不同浓度的苦瓜提取物灌胃组,灌胃质量分数分别为13.5、27和54 g/kg。以10周为期,每天以同等体积流质灌胃两次,其中,正常对照组和模型组用w=0.5%羧甲基纤维素钠灌胃,药物组用不同浓度的苦瓜提取物灌胃。记录小鼠每日食物摄入量和每周体质量。同时测定内脏白色脂肪组织质量、血清甘油三酯(TG)、胆固醇(CHO)、低密度脂蛋白胆固醇(LDLC)、高密度脂蛋白胆固醇(HDL-C)、血糖和胰岛素水平,最后根据HOMA法计算胰岛素抵抗指数,HE染色确定附睾白色脂肪组织的脂肪细胞是否肥大。结果显示,54 g/kg苦瓜提取物组对小鼠摄食无影响,但小鼠体质量、附睾和内脏白色脂肪组织总质量减少,该剂量的苦瓜提取物显著抑制被提升的血清TG、LDL-C、葡萄糖、胰岛素浓度和胰岛素抵抗指数;而27 g/kg苦瓜提取物则显著降低TG浓度;13.5、27和54 g/kg苦瓜提取物分别显著升高HDL-C水平;苦瓜提取物能剂量依赖性地减轻高脂饮食诱导的脂肪细胞肥大。In order to investigate the protective effects of Momordica charantia extract（ MCE） on obesity and insulin resistance,C57 BL /6j mice fed in a high-fat diet（ HFD） were used as animal model. Obese mouse model was successfully made after 2-week HFD induction. The mice were then randomly divided into normal control,model control,MCE dose of 13. 5,27. 0,and 54. 0 g / kg treated groups. Mice in normal and model control groups were treated with 0. 5% CMC-Na,and other groups were treated with MCE（ i. g.）,respectively,with the same volume twice a day for ten weeks. It was shown that the body weight,epididymal and total visceral white adipose tissue weight were decreased by treatment of MCE 54. 0 g / kg without the change of food intake. The increased serum TG,CHO,LDL-C,glucose,insulin concentration and insulin resistance index were significantly inhibited. The concentration of TG was also decreased by the treatment of MCE 27. 0 g / kg; while the concentration of HDL-C was increased by the treatment of MCE 13. 5,27. 0 and 54. 0 g / kg,respectively. The adipocyte hypertrophy induced by HFD was mitigated by MCE dose-dependently.

关 键 词：苦瓜 高脂喂食 糖尿病 胰岛素抵抗 肥胖 

分 类 号：R961[医药卫生—药理学]