基于环介导等温扩增技术检测瓜类细菌性果斑病菌  

Rapid diagnostic methods for Acidovorax citrulli using LAMP

作　　者：赵玉强[1] 田艳丽[2] 罗金燕[1] 姚红梅[1] 余慧[1] 陈磊[1] 胡白石[2] 

机构地区：[1]上海市农业技术推广服务中心,上海201103 [2]南京农业大学植物保护学院,农作物生物灾害综合治理教育部重点实验室,南京210095

出　　处：《植物保护》2015年第5期116-120,共5页Plant Protection

基　　金：上海市农业技术推广服务中心科技发展基金(3031344);国家“863”项目(2012AA101501);上海市科技兴农重点攻关项目[沪农科攻字(2014)第7-3-1号,沪农科攻字(2012)第2-10号]

摘　　要：本文利用环介导等温扩增技术(loop-mediated isothermal amplification,LAMP),针对西瓜嗜酸菌(Acidovorax citrulli)基因组中Aave_4063和Aave_4064序列设计了2对特异引物Ac-F3/Ac-B3和Ac-FIP/Ac-BIP,建立了西瓜嗜酸菌的LAMP检测体系。利用该体系在65℃保温1h,通过荧光显色即可完成检测。设计的引物特异性强,其检测灵敏度为2.0×101 cfu/mL。该方法为西瓜嗜酸菌的检疫及其所致病害的快速诊断提供了新的技术。The loop-mediated isothermal amplification （LAMP）was established for rapid detection of Acidovorax citrul-li .Four primers,Ac-F3/Ac-B3 and Ac-FIP/Ac-BIP,were designed specifically to recognize the Aave _4063 and Aave _4064 genes of A .citrulli .The LAMP products was obtained under isothermal conditions at 65 ℃ for 1 h.The result showed that no amplicon was observed except A .citrulli strains.The detection limits of pure cultures were 2.0×10^1 cfu/mL.This LAMP detection system provides a new alternative method for the rapid detection of A .citrulli .

关 键 词：环介导等温扩增技术 瓜类细菌性果斑病菌 检测 

分 类 号：S436.5[农业科学—农业昆虫与害虫防治]