引起大豆苗期根腐病的大豆拟茎点种腐病菌鉴定  

Identification of Phomopsis longicolla causing soybean root rot at seedling stage

作　　者：耿肖兵 王春玲[1] 黄铭慧 李永刚[1] 

机构地区：[1]东北农业大学农学院,哈尔滨150030

出　　处：《植物保护》2015年第5期127-129,144,共4页Plant Protection

基　　金：东北农业大学大豆生物学教育部重点实验室开放基金(SB12B01)

摘　　要：对10个引起大豆根腐病的真菌分离物进行鉴定。结果表明,这些分离物形态学上与大豆拟茎点种腐病菌相似。用ITS通用引物进行PCR扩增,该菌株与拟茎点种腐病菌MP4PL11PS(GenBank登录号HQ130441.1)同源性达98%。因此,确定该菌株为大豆拟茎点种腐病菌。The 10 fungal isolates causing soybean root rot were identified.The results showed that 10 isolates had similarity to Phomopsis longicolla in morphology.BLAST search results showed that the sequence of P .longicolla strain MP4PL1 1PS （GenBank accession number HQ130441.1）was homologous to the tested strain with a similari-ty of 98% by using universal primers （ITS）for amplification.So,the isolated strain was identified as P .longicolla .

关 键 词：根腐病 大豆拟茎点种腐病菌 大豆 苗期 鉴定 

分 类 号：S435.2[农业科学—农业昆虫与害虫防治]