354份小麦种质中抗白粉病基因Pm21和Pm13的分布研究  

Distribution of powdery mildew resistance gene Pm21 and Pm13 among 354 wheat germplasms

作　　者：董娜[1] 李淦[1] 张亚娟[1] 任翠翠[1] 茹振钢[1] 

机构地区：[1]河南科技学院小麦中心,河南省高等学校作物分子育种重点开放实验室,现代生物育种河南省协同创新中心,新乡453003

出　　处：《植物保护》2015年第5期164-168,共5页Plant Protection

基　　金：国家科技支撑计划(2011BAD07B02);河南省教育厅科学技术研究重点项目(12B210003)

摘　　要：为了解本课题组现有种质资源中抗白粉病基因Pm21和Pm13的分布情况,本研究利用共分离分子标记SCAR1400和SCAR564,对收集于全国多个育种单位的354份小麦种质进行检测。结果表明:在检测的354份种质中,携带Pm21的有21份,分别为‘石H083-366’、‘BH50890-1-1’、‘06Y06’、‘南农02Y393’、‘09P80’、‘09P131’、‘09P283’、‘黔麦18号’、‘黔早麦15-3’、‘黔979-1’、‘黔9963-3’、‘黔9961-2’、‘YN06’、‘绵杂麦168’、‘MR168’、‘09品A6’、‘09品E6’、‘内麦836’、‘内麦9号’、‘绵阳39’和‘绵麦367’,检出率为5.9%,其中,8份来源于四川,来源于江苏和贵州的各有5份,河北、陕西和云南的材料中各有1份;仅有1份种质‘中大01’携带Pm13,来源于河南,检出率为0.28%。本研究结果可为抗白粉病基因资源的有效利用提供重要参考依据。To understand the distribution of powdery mildew resistance gene Pm 21 and Pm 13 among 354 wheat germplasm resources collected from many breeding units all over the country and kept in wheat research center of Henan Institute of Science and Technology,their co-segregated molecular markers SCAR 1 400 and SCAR 5 64 were used to assist detection in this study.The results showed that 21 wheat germplasms among the 354 germplasm re-sources were confirmed to carry the powdery mildew resistance gene Pm 21,including ‘Shi H083-366’,‘BH50890-1-1’,‘06Y06’,‘Nannong 02Y393’,‘09P80’,‘09P131’,‘09P283’,‘Qianmai 18’,‘Qianzaomai 1 5-3’,‘Qian 979-1’,‘Qian 9963-3’,‘Qian 9961-2’,‘YN06’,‘Mianzamai 1 68’,‘MR1 68’,‘09 pin A6’,‘09 pin E6’,‘Nei-mai 836’,‘Neimai 9’,‘Mianyang 39’and ‘Mianmai 367’.The positive rate was 5.9%.Among these Pm 21-pos-itive germplasms,eight were collected from Sichuan Province,five from Guizhou and Jiangsu,and one from He-bei,Shanxi and Yunnan.Additionally,only one germplasm named ‘Zhongda 01’was confirmed to carry Pm 13, which was collected from Henan Province.The Pm 13-positive rate was 0.28%.The results of this study may pro-vide important information for effective utilization of the powdery mildew resistance gene resources.

关 键 词：小麦 白粉病 PM21 Pm13 

分 类 号：S435.121.46[农业科学—农业昆虫与害虫防治]