愈痫灵方对KA致痫大鼠海马及颞叶皮质多药耐药基因MDR1b表达的影响  

Effects of Yuxianling Decoction on the Expression of Multiple Drug Resistant Gene MDR1b in Hippocampusand Temporal Lobe Cortex of Epileptic Rats Induced by Kainic Acid

作　　者：李振光[1] 宋祖丽 王净净[3] 李智雄[3] 谢静涛[3] 左亚杰[4] 张曦[3] 肖瑶[3] 

机构地区：[1]湖南省脑科医院,湖南长沙410007 [2]湖南中医药大学第二附属医院,湖南长沙410005 [3]湖南中医药大学,湖南长沙410208 [4]湖南中医药大学第一附属医院,湖南长沙410007

出　　处：《湖南中医药大学学报》2015年第11期18-21,共4页Journal of Hunan University of Chinese Medicine

基　　金：湖南省科学技术厅科技计划一般项目(2012SK3132);湖南省教育厅重点科研课题(11A084)

摘　　要：目的探讨愈痫灵方对耐药性癫痫模型大鼠海马及颞叶皮质多药耐药基因MDR1b表达的影响。方法 1造模:在脑立体定位仪引导下,海马区微量进样器注入红藻氨酸(KA)1.0μL(浓度为1.0μg/μL)点燃造模,选取发作行为级别在Racines标准Ⅳ级以上者,以丙戊酸钠及卡马西平灌胃干预14 d,再次亚惊厥剂量0.5μL KA复燃,选择再次发作级别在Ⅳ级以上或持续状态大鼠,且脑电图检测有癫痫样波发放者筛选为造模成功的耐药难治性癫痫模型鼠。2分组与处理:造模成功鼠随机分为愈痫灵干预组、拉莫三嗪对照组、模型组,另设假手术对照组、空白对照组共计5组,每组12只。分别给予愈痫灵汤剂、拉莫三嗪及同等体积的蒸馏水(2 m L/d)灌胃30 d。3标本处置与检测:采用荧光实时定量聚合酶链反应(RT-PCR)的方法,分别检测海马区与颞叶皮层MDR1b的表达。结果与假手术对照组、空白对照组间比较,造模成功组海马区MDR1b的表达水平明显升高(P<0.01),颞叶皮质区差异无统计学意义(P>0.05);所有造模组的海马区MDR1b表达高于颞叶皮质,差异有统计学意义(P<0.01)。与模型组比较,愈痫灵、拉莫三嗪干预后能显著降低KA致痫鼠海马区MDR1b的表达水平(P<0.05);愈痫灵方组与拉莫三嗪组间比较,海马与颞叶皮质之间差异均无统计学意义(P>0.05);各组间颞叶皮质区MDR1b表达差异无统计学意义(P>0.05)。结论 KA造模耐药性癫痫大鼠模型海马区多药耐药基因MDR1b的表达较皮质区明显升高。愈痫灵方降低海马区MDR1b的表达,可能是其抗耐药性癫痫作用机制之一。Objective To investigate the effects of Yuxianling Decoction （YXLD） on expression of multiple drug resistant gene MDR^b in hippocampus and temporal lobe cortex of epileptic rats induced by kaicic acid （KA）. Methods （1） Making models：The Hippocampus in rats located by brain stereotactic apparatus was microinjected 1μg KA （1.0 μg/μL） to kindle seizures models. The rats at above seizure behavior surpass 1V level were intragastric administration interfered by sodium valproate and carbamazepine for 14 days, and rekindled 0.5 μL. The rats second attacked seizure at above 1V level and with persistent state were selected.Moreover, the rats with epileptiform discharge wave were selected to be successful resistant refractory epilepsy model rats. （2） Grouping and treatment： The Successful model rats were randomly divided into YXLD group, lamotrigine control group and model group, rats were also assigned into sham operation group and normal control group, 12 rats in each group. The rats were intragastric administration interfered by the same volume of distilled water, lamotrigine and YXLD respectively for 30 days （2 mL/d）. （3） Selecting and detecting specimens： The expression of MDRlb in hippocampus area and temporal lobe cortex was detected by real-time fluorescent quantitative polymerase chain reaction （RT-PCR） method. Results Compared with sham operation control group and normal blank control group, the expression level of MDRlb in hippocampus area was increased obviously （P〈0.01）, the differences in temporal lobe cortex have no statistical significance （P〉0.05）; The MDR1b expression in hippocampus area of all model groups was higher than that in temporal lobe cortex, the differences have statistical significance （P〈0.05）. Compared with the model group, lamotrigine and YXLD intervention could reduce MDRlb expression level in hippocampus area of KA epileptic rats （P〈0.05）; Between the YXLD group and lamotriginegroup, the differences in hippocampus and tempor

关 键 词：耐药性癫痫 愈痫灵方 多药耐药基因MDR1b 红藻氨酸 

分 类 号：R285.5[医药卫生—中药学] R742.1[医药卫生—中医学]